ArticleMolecular biology of the cell1996
Signals from the spindle midzone are required for the stimulation of cytokinesis in cultured epithelial cells.
Article in Molecular biology of the cell, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
71 citing papers in PubMed, 186 citations in OpenAlex.
- Physical Frameworks and Theories on How Autonomous Biological Oscillators Synchronize.Advances in experimental medicine and biology · 2026Review
- A cortical pool of LIN-5 (NuMA) controls cytokinetic furrow formation and cytokinesis completion.The Journal of cell biology · 2025Article
- Kinesin KIFC3 is essential for microtubule stability and cytokinesis in oocyte meiosis.Cell communication and signaling : CCS · 2024Article
- Article
- Lateral and longitudinal compaction of PRC1 overlap zones drives stabilization of interzonal microtubules.Molecular biology of the cell · 2023Article
- Centralspindlin-mediated transport of RhoGEF positions the cleavage plane for cytokinesis.Science signaling · 2023Article
- Functional midbody assembly in the absence of a central spindle.The Journal of cell biology · 2022Article
- Review
- Molecular Mechanism of Cytokinesis.Annual review of biochemistry · 2019Review
- PLK1 plays dual roles in centralspindlin regulation during cytokinesis.The Journal of cell biology · 2019Article
- Compressive Force Spectroscopy: From Living Cells to Single Proteins.International journal of molecular sciences · 2018Review
- LET-99 functions in the astral furrowing pathway, where it is required for myosin enrichment in the contractile ring.Molecular biology of the cell · 2017Article
- Vertebrate Embryonic Cleavage Pattern Determination.Advances in experimental medicine and biology · 2017Review
- Augmin shapes the anaphase spindle for efficient cytokinetic furrow ingression and abscission.Molecular biology of the cell · 2016Article
- Splitting the cell, building the organism: Mechanisms of cell division in metazoan embryos.IUBMB life · 2015Review
- An astral simulacrum of the central spindle accounts for normal, spindle-less, and anucleate cytokinesis in echinoderm embryos.Molecular biology of the cell · 2014Article
- Orbit/CLASP is required for myosin accumulation at the cleavage furrow in Drosophila male meiosis.PloS one · 2014Article
- SHCBP1 is required for midbody organization and cytokinesis completion.Cell cycle (Georgetown, Tex.) · 2014Article
- Binding of the CYK-4 subunit of the centralspindlin complex induces a large scale conformational change in the kinesin subunit.The Journal of biological chemistry · 2013Article
- Anterior PAR proteins function during cytokinesis and maintain DYN-1 at the cleavage furrow in Caenorhabditis elegans.Cytoskeleton (Hoboken, N.J.) · 2012Article
11 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The interaction between the mitotic spindle and the cellular cortex is thought to play a critical role in stimulating cell cleavage. However, little is understood about the nature of such interactions, particularly in tissue culture cells. We have investigated the role of the spindle midzone in signaling cytokinesis by creating a barrier in cultured epithelial cells with a blunted needle, to block signals that may emanate from this region. When the barrier was created during metaphase or early anaphase, cleavage took place only on the sides of the cortex facing the mitotic spindle. Microtubules on the cleaving side showed organization typical of that in normal dividing cells. On the noncleaving side, most microtubules passed from one side of the equator into the other without any apparent organization, and actin filaments failed to organize in the equatorial region. When the barrier was created after the first minute of anaphase, cells showed successful cytokinesis, with normal organization of microtubules and actin filaments on both sides of the barrier. Our study suggests that transient signals from the midzone of early anaphase spindles are required for equatorial contraction in cultured cells and that such signaling may involve the organization of microtubules near the equator.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.