ArticleThe Biochemical journal1996
Phospholipase C in mouse oocytes: characterization of beta and gamma isoforms and their possible involvement in sperm-induced Ca2+ spiking.
Article in The Biochemical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- ZneLife · 2023Article
- ZnbioRxiv : the preprint server for biology · 2023Article
- Dual-FRET imaging of IPScientific reports · 2019Article
- Article
- Signal transduction in mammalian oocytes during fertilization.Cell and tissue research · 2016Review
- Whole-genome resequencing analyses of five pig breeds, including Korean wild and native, and three European origin breeds.DNA research : an international journal for rapid publication of reports on genes and genomes · 2015Article
- Biochemical and genetic evidence for the presence of multiple phosphatidylinositol- and phosphatidylinositol 4,5-bisphosphate-specific phospholipases C in Tetrahymena.Eukaryotic cell · 2011Article
- Impact of marine drugs on animal reproductive processes.Marine drugs · 2009Review
- The roles of Ca2+, downstream protein kinases, and oscillatory signaling in regulating fertilization and the activation of development.Developmental biology · 2008Review
- Alterations of PLCbeta1 in mouse eggs change calcium oscillatory behavior following fertilization.Developmental biology · 2007Article
- Proteolytic processing of phospholipase Czeta and [Ca2+]i oscillations during mammalian fertilization.Developmental biology · 2007Article
- Calcium at fertilization and in early development.Physiological reviews · 2006Review
- The biological significance of phospholipase C beta 1 gene mutation in mouse sperm in the acrosome reaction, fertilization, and embryo development.Journal of assisted reproduction and genetics · 2001Article
- Cytoplasmic and nuclear phospholipase C-beta 1 relocation: role in resumption of meiosis in the mouse oocyte.Molecular biology of the cell · 2000Article
- Different Ca2+-releasing abilities of sperm extracts compared with tissue extracts and phospholipase C isoforms in sea urchin egg homogenate and mouse eggs.The Biochemical journal · 2000Article
- The soluble sperm factor that causes Ca2+ release from sea-urchin (Lytechinus pictus) egg homogenates also triggers Ca2+ oscillations after injection into mouse eggs.The Biochemical journal · 1999Article
- Involvement of phospholipase Cgamma1 in mouse egg activation induced by a truncated form of the C-kit tyrosine kinase present in spermatozoa.The Journal of cell biology · 1998Article
- Calcium release at fertilization in starfish eggs is mediated by phospholipase Cgamma.The Journal of cell biology · 1997Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study involved an investigation of the role of phospholipase C (PLC) in generating repetitive Ca2+ spikes at fertilization. Using a PCR-based strategy we have demonstrated that mouse oocytes have mRNA coding for PLC beta 1, PLC beta 3 and PLC gamma isoenzymes. Furthermore, immunodetection of PLC gamma 1 using monoclonal antibodies reveals that PLC gamma 1 protein is present in mature mouse oocytes, ruling out the possibility that mRNA was being transcribed but not expressed. We were unsuccessful at detecting the presence of PLC beta protein, but the presence of this isoform can be inferred from functional studies. The PLC inhibitor, U73122, exerted an inhibitory effect on oocytes activated by spermatozoa or acetylcholine at concentrations of 10 and 30 microM respectively, while its inactive analogue had no effect. The soluble tyrosine kinase inhibitors, genistein (100 microM), herbimycin (10 microM) and geldanamycin (0.6 microM) which could affect signalling through PLC gamma hindered but never completely inhibited Ca2+ spiking in response to fertilization. We conclude that the activation of PLC to generate InsP3 may play a critical role in fertilization.
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