ArticleThe Journal of physiology1996
Activation of L-arginine transport (system y+) and nitric oxide synthase by elevated glucose and insulin in human endothelial cells.
Article in The Journal of physiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.
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Who cites it
35 citing papers in PubMed, 1 synthesis or guideline pooled it, 155 citations in OpenAlex.
- Tight glycemic control does not affect asymmetric-dimethylarginine in septic patients.Intensive care medicine · 2008Pooled it
- A maternal meal affects clinical Doppler parameters in the fetal middle cerebral artery.PloS one · 2018Trial
- Potential role of A2B adenosine receptors on proliferation/migration of fetal endothelium derived from preeclamptic pregnancies.BioMed research international · 2014Trial
- Impact of 50 g OGTT-Induced Acute Hyperglycemia on Uteroplacental and Fetoplacental Circulations in Gestational Diabetes Screening: A Prospective Single-Center Study.Journal of clinical medicine · 2025Article
- Effect of propolis and honey in hyperglycemia-induced kidney and liver injuries, proteinuria, and oxidant and antioxidant parameters.World journal of experimental medicine · 2025Article
- Physiological and Pathophysiological Role of Large-Conductance Calcium-Activated Potassium Channels (BKCa) in HUVECs and Placenta.Advances in experimental medicine and biology · 2023Review
- Review
- Exogenous carbohydrate and regulation of muscle carbohydrate utilisation during exercise.European journal of applied physiology · 2021Review
- Arginine and Endothelial Function.Biomedicines · 2020Review
- Essential hypertension: A filtered serum based metabolomics study.Scientific reports · 2017Article
- Modulating DDAH/NOS Pathway to Discover Vasoprotective Insulin Sensitizers.Journal of diabetes research · 2016Review
- Insulin Induces Relaxation and Decreases Hydrogen Peroxide-Induced Vasoconstriction in Human Placental Vascular Bed in a Mechanism Mediated by Calcium-Activated Potassium Channels and L-Arginine/Nitric Oxide Pathways.Frontiers in physiology · 2016Article
- Cardiovascular Action of Insulin in Health and Disease: Endothelial L-Arginine Transport and Cardiac Voltage-Dependent Potassium Channels.Frontiers in physiology · 2016Review
- Insulin reverses D-glucose-increased nitric oxide and reactive oxygen species generation in human umbilical vein endothelial cells.PloS one · 2015Article
- Impaired adenosine-mediated angiogenesis in preeclampsia: potential implications for fetal programming.Frontiers in pharmacology · 2014Review
- Metabolic actions of angiotensin II and insulin: a microvascular endothelial balancing act.Molecular and cellular endocrinology · 2013Review
- Hypertension in metabolic syndrome: vascular pathophysiology.International journal of hypertension · 2013Article
- Serum from calorie-restricted rats activates vascular cell eNOS through enhanced insulin signaling mediated by adiponectin.PloS one · 2012Article
- Insulin rapidly stimulates L-arginine transport in human aortic endothelial cells via Akt.Biochemical and biophysical research communications · 2011Article
- Antihypertensive effects of dietary protein and its mechanism.The International journal of angiology : official publication of the International College of Angiology, Inc · 2010Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
1. Modulation of L-arginine transport (system y+) and release of nitric oxide (NO) and prostacyclin (PGI2) by elevated glucose and insulin were investigated in human cultured umbilical vein endothelial cells. 2. Elevated glucose induced a time- (6-12 h) and concentration-dependent stimulation of L-arginine transport, which was reversible and associated with a 3-fold increase in intracellular cGMP accumulation (index of NO synthesis) and 75% decrease in PGI2 production. 3. Elevated glucose had no effect on the initial transport rates for L-serine, L-citrulline, L-leucine, L-cystine or 2-deoxyglucose. 4. Resting membrane potential was unaffected by elevated glucose whereas basal intracellular [Ca2+] increased from 65 +/- 5 nM to 136 +/- 16 nM. 5. Insulin induced a protein synthesis-dependent stimulation of L-arginine transport and increased NO and PGI2 production in cells exposed to 5 mM glucose. 6. In cells exposed to high glucose, insulin downregulated elevated rates of L-arginine transport and cGMP accumulation but had no effect on the depressed PGI2 production. 7. Our findings suggest that insulin's normal stimulatory action on human endothelial cell vasodilator pathways may be impaired under conditions of sustained hyperglycaemia.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.