ArticleThe Journal of cell biology1996
Active transport of photoactivated tubulin molecules in growing axons revealed by a new electron microscopic analysis.
Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 39 citations in OpenAlex.
- One gene, many phenotypes: the role of KIF5A in neurodegenerative and neurodevelopmental diseases.Cell communication and signaling : CCS · 2025Review
- KIFC3, a microtubule minus end-directed motor for the apical transport of annexin XIIIb-associated Triton-insoluble membranes.The Journal of cell biology · 2001Article
- Rapid intermittent movement of axonal neurofilaments observed by fluorescence photobleaching.Molecular biology of the cell · 2001Article
- Tubulin and neurofilament proteins are transported differently in axons of chicken motoneurons.Cellular and molecular neurobiology · 2000Article
- Glutamate slows axonal transport of neurofilaments in transfected neurons.The Journal of cell biology · 2000Article
- Kinesin superfamily protein 3 (KIF3) motor transports fodrin-associating vesicles important for neurite building.The Journal of cell biology · 2000Article
- Reorganization and movement of microtubules in axonal growth cones and developing interstitial branches.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- Slow transport of unpolymerized tubulin and polymerized neurofilament in the squid giant axon.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- STOP proteins are responsible for the high degree of microtubule stabilization observed in neuronal cells.The Journal of cell biology · 1998Article
- Transport and turnover of microtubules in frog neurons depend on the pattern of axonal growth.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- Identification of a microtubule-associated motor protein essential for dendritic differentiation.The Journal of cell biology · 1997Article
- Microtubule transport from the cell body into the axons of growing neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1997Article
- Delivery of newly synthesized tubulin to rapidly growing distal axons of sympathetic neurons in compartmented cultures.The Journal of cell biology · 1996Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To determine whether tubulin molecules transported in axons are polymers or oligomers, we carried out electron microscopic analysis of the movement of the tubulin molecules after photoactivation. Although previous optical microscopic analyses after photobleaching or photoactivation had suggested that most of the axonal microtubules were stationary, they were not sufficiently sensitive to allow detection of actively transported tubulin molecules which were expected to be only a small fraction of total tubulin molecules in axons. In addition, some recent studies using indirect approaches suggested active polymer transport as a mechanism for tubulin transport (Baas, P.W., F.J. Ahmad. 1993. J. Cell Biol. 120:1427-1437; Yu, W., V.E. Centonze, F.J. Ahmad, and P.W. Bass, 1993, J. Cell Biol. 122:349-359; Ahmad, F.J., and P.W. Bass. 1995. J. Cell Sci. 108:2761-2769). So, whether transported tubulin molecules are polymers or not remain to be determined. To clear up this issue, we made fluorescent marks on the tubulin molecules in the axons using a photoactivation technique and performed electron microscopic immunocytochemistry using anti-fluorescein antibody. Using this new method we achieved high resolution and high sensitivity for detecting the transported tubulin molecules. In cells fixed after permeabilization, we found no translocated microtubules. In those fixed without permeabilization, in which oligomers and heterodimers in addition to polymers were preserved, we found much more label in the regions distal to the photoactivated regions than in the proximal regions. These data indicated that tubulin molecules are transported not as polymers but as heterodimers or oligomers by an active mechanism rather than by diffusion.
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