ArticleThe Journal of cell biology1996
Oligomerization and maturation of Na,K-ATPase: functional interaction of the cytoplasmic NH2 terminus of the beta subunit with the alpha subunit.
Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Association between Decreased SGK1 and Increased Intestinal α-Synuclein in an MPTP Mouse Model of Parkinson's Disease.International journal of molecular sciences · 2023Article
- Na,K-ATPase α1 and β-subunits show distinct localizations in the nervous tissue of the large milkweed bug.Cell and tissue research · 2022Article
- Genetically altered animal models for ATP1A3-related disorders.Disease models & mechanisms · 2021Article
- The Physiology of the Gastric Parietal Cell.Physiological reviews · 2020Review
- Human Mutations in SLC2A9 (Glut9) Affect Transport Capacity for Urate.Frontiers in physiology · 2018Article
- Mineralocorticoids modulate the expression of the β-3 subunit of the NaChannels (Austin, Tex.) · 2017Article
- FXYD8, a Novel Regulator of Renal NaFrontiers in physiology · 2017Article
- Ouabain affects cell migration via Na,K-ATPase-p130cas and via nucleus-centrosome association.PloS one · 2017Article
- Tuning of the Na,K-ATPase by the beta subunit.Scientific reports · 2016Article
- Downregulation of TGF-β Receptor-2 Expression and Signaling through Inhibition of Na/K-ATPase.PloS one · 2016Article
- Genetic effects of ATP1A2 in familial hemiplegic migraine type II and animal models.Human genomics · 2013Review
- Ouabain-induced apoptosis in cochlear hair cells and spiral ganglion neurons in vitro.BioMed research international · 2013Article
- Intracellular trafficking of FXYD1 (phospholemman) and FXYD7 proteins in Xenopus oocytes and mammalian cells.The Journal of biological chemistry · 2012Article
- Cyclophilin B interacts with sodium-potassium ATPase and is required for pump activity in proximal tubule cells of the kidney.PloS one · 2010Article
- The polarized distribution of Na+,K+-ATPase: role of the interaction between {beta} subunits.Molecular biology of the cell · 2010Article
- E2P state stabilization by the N-terminal tail of the H,K-ATPase beta-subunit is critical for efficient proton pumping under in vivo conditions.The Journal of biological chemistry · 2009Article
- Inter-subunit interaction of gastric H+,K+-ATPase prevents reverse reaction of the transport cycle.The EMBO journal · 2009Article
- Expression and trafficking of the gamma subunit of Na,K-ATPase in hypertonically challenged IMCD3 cells.Acta histochemica et cytochemica · 2008Article
- Identification and expression analysis of genes associated with bovine blastocyst formation.BMC developmental biology · 2007Article
- Janus model of the Na,K-ATPase beta-subunit transmembrane domain: distinct faces mediate alpha/beta assembly and beta-beta homo-oligomerization.Journal of molecular biology · 2007Article
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7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Subunit assembly plays an essential role in the maturation of oligomeric proteins. In this study, we have characterized the main structural and functional consequences of the assembly of alpha and beta subunits of Na,K-ATPase. Xenopus oocytes injected with alpha and/or beta cRNA were treated with brefeldin A, which permitted the accumulation of individual subunits or alpha-beta complexes in the ER. Only alpha subunits that are associated with beta subunits become resistant to trypsin digestion and cellular degradation. Similarly, assembly with beta subunits is necessary and probably sufficient for the catalytic alpha subunit to acquire its main functional properties at the level of the ER, namely the ability to adopt different ligand-dependent conformations and to hydrolyze ATP in an Na(+)- and K(+)-dependent, ouabain-inhibitable fashion. Not only the alpha but also the beta subunit undergoes a structural change after assembly, which results in a global increase in its protease resistance. Furthermore, extensive and controlled proteolysis assays on wild-type and NH2-terminally modified beta subunits revealed a K(+)-dependent interaction of the cytoplasmic NH2 terminus of the beta subunit with the alpha subunit, which is likely to be involved in the modulation of the K(+)-activation of the Na,K-pump transport activity. Thus, we conclude that the ER assembly process not only establishes the basic structural interactions between individual subunits, which are required for the maturation of oligomeric proteins, but also distinct, functional interactions, which are involved in the regulation of functional properties of mature proteins.
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