Evidence map›Paper›PMID 8682857›Full record

ArticleThe Journal of cell biology1996

A biosynthetic regulated secretory pathway in constitutive secretory cells.

R A Chavez, S G Miller, H P Moore

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed
4.2field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed, 90 citations in OpenAlex.

  1. Trial
  2. A Compendium of Information on the Lysosome.Frontiers in cell and developmental biology · 2021
    Review
  3. Article
  4. Article
  5. Review
  6. Article
  7. Review
  8. Article
  9. Article
  10. Review
  11. Lysosomes are the major vesicular compartment undergoing Ca2+-regulated exocytosis from cortical astrocytes.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008
    Article
  12. Resolving vesicle fusion from lysis to monitor calcium-triggered lysosomal exocytosis in astrocytes.Proceedings of the National Academy of Sciences of the United States of America · 2007
    Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Review
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

R A ChavezUniversity of California at Berkeley, Department of Molecular & Cell Biology 94720-3200, USA.
S G Miller
H P Moore
University of California, Berkeley · US

Funding

SORTING OF PROTEINS IN REGULATED SECRETORY CELLSR01GM035239 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI MOORE, HSIAO-PING H · 1985 to 1996
–
NIGMS NIH HHS GM 35239
6 · The paper itself

Abstract

It has frequently been proposed that while the constitutive secretory pathway is present in all cells, the regulated secretory pathway is found only in specialized cells such as neuronal, endocrine, or exocrine types. In this study we provide evidence that suggests that this distinction is not as restrictive as proposed. We have identified a population of post-Golgi storage vesicles in several constitutive secretory cells using [35S]SO4-labeled glycosaminoglycan (GAG) chains as a marker. A fraction of this pool of vesicles can undergo exocytosis in response to stimuli such as cytoplasmic Ca2+ and phorbol esters. The effect of Ca2+ was demonstrated both in intact cells in the presence of the ionophore A23187 and in streptolysin-O-permeabilized semi-intact cells. N-ethylmaleiimide, under conditions known to block regulated and constitutive secretion, inhibited the stimulated secretion from these cells, suggesting that the observed release of labeled GAG chains was not due to a leakage artefact. Subcellular fractionation revealed that the stored GAG chains were in low-density membrane granules (d approximately 1.12 g/ml), whose size was greater than that of synaptic-like vesicles found in PC12 cells. In addition, in CHO cells that express epitope-tagged rab 3D, the labeled GAG chains were found to cofractionate with the exogenous rab protein. When expressed in the regulated cell line AtT-20, this tagged rab protein was found to colocalize with ACTH-containing dense-core granules by indirect immunofluorescence. Taken together, these results provide evidence for the presence of a cryptic regulated secretory pathway in "constitutive" cells and suggest that the regulated secretory pathway is more widespread amongst different cell types than previously believed.

Indexed as

AnimalsBiological TransportCalciumCell LineCHO CellsCricetinaeCytoplasmic GranulesEthylmaleimideExocytosisFemaleGene ExpressionGlycosaminoglycansGlycosidesGolgi ApparatusGTP-Binding ProteinsL CellsCalciumEthylmaleimideGlycosaminoglycansGlycosidesGTP-Binding Proteinsrab3 GTP-Binding ProteinsRNA, Messengerxylosides

Identifiers

PMID8682857
PMCPMC2120905
OpenAlexW2092488957

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.