ArticleJournal of bacteriology1996
Identification of a major cis-acting DNA element controlling the bidirectionally transcribed penicillin biosynthesis genes acvA (pcbAB) and ipnA (pcbC) of Aspergillus nidulans.
Article in Journal of bacteriology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 69 citations in OpenAlex.
- Identification of a Bidirectional Promoter fromJournal of fungi (Basel, Switzerland) · 2022Article
- Article
- Comparative genomics of Alexander Fleming's original Penicillium isolate (IMI 15378) reveals sequence divergence of penicillin synthesis genes.Scientific reports · 2020Article
- Functional characterization of an inducible bidirectional promoter from Fusarium oxysporum f. sp. cubense.Scientific reports · 2020Article
- The putative histone-like transcription factor FgHltf1 is required for vegetative growth, sexual reproduction, and virulence in Fusarium graminearum.Current genetics · 2019Article
- Induction of Mitochondrial Reactive Oxygen Species Production by Itraconazole, Terbinafine, and Amphotericin B as a Mode of Action against Aspergillus fumigatus.Antimicrobial agents and chemotherapy · 2017Article
- Penicillin production in industrial strain Penicillium chrysogenum P2niaD18 is not dependent on the copy number of biosynthesis genes.BMC biotechnology · 2017Article
- Distinct amino acids of histone H3 control secondary metabolism in Aspergillus nidulans.Applied and environmental microbiology · 2013Article
- Bacteria-induced natural product formation in the fungus Aspergillus nidulans requires Saga/Ada-mediated histone acetylation.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Transcriptional regulatory elements in fungal secondary metabolism.Journal of microbiology (Seoul, Korea) · 2011Review
- The CCAAT-binding complex coordinates the oxidative stress response in eukaryotes.Nucleic acids research · 2010Article
- Aspergillus nidulans natural product biosynthesis is regulated by mpkB, a putative pheromone response mitogen-activated protein kinase.Applied and environmental microbiology · 2008Article
- Interaction of HapX with the CCAAT-binding complex--a novel mechanism of gene regulation by iron.The EMBO journal · 2007Article
- Protein kinase C (PkcA) of Aspergillus nidulans is involved in penicillin production.Applied and environmental microbiology · 2006Article
- Cloning and expression analysis of the pcbAB-pcbC beta-lactam genes in the marine fungus Kallichroma tethys.Applied and environmental microbiology · 2003Article
- Aspergillus enzymes involved in degradation of plant cell wall polysaccharides.Microbiology and molecular biology reviews : MMBR · 2001Review
- Molecular control of expression of penicillin biosynthesis genes in fungi: regulatory proteins interact with a bidirectional promoter region.Journal of bacteriology · 2000Review
- Cloning and characterization of an Aspergillus nidulans gene involved in the regulation of penicillin biosynthesis.Applied and environmental microbiology · 1999Article
- AnCF, the CCAAT binding complex of Aspergillus nidulans, contains products of the hapB, hapC, and hapE genes and is required for activation by the pathway-specific regulatory gene amdR.Molecular and cellular biology · 1999Article
- Molecular regulation of beta-lactam biosynthesis in filamentous fungi.Microbiology and molecular biology reviews : MMBR · 1998Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The beta-lactam antibiotic penicillin is produced as a secondary metabolite by some filamentous fungi. In this study, the molecular regulation of the Aspergillus (Emericella) nidulans penicillin biosynthesis genes acvA (pcbAB) and ipnA (pcbC) was analyzed. acvA and ipnA are divergently oriented and separated by an intergenic region of 872 bp. Translational fusions of acvA and ipnA with the two Escherichia coli reporter genes lacZ and uidA enabled us to measure the regulation of both genes simultaneously. A moving-window analysis of the 872-bp intergenic region indicated that the divergently oriented promoters are, at least in part, overlapping and share common regulatory elements. Removal of nucleotides -353 to -432 upstream of the acvA gene led to a 10-fold increase of acvA-uidA expression and simultaneously to a reduction of ipnA-lacZ expression to about 30%. Band shift assays and methyl interference analysis using partially purified protein extracts revealed that a CCAAT-containing DNA element within this region was specifically bound by a protein (complex), which we designated PENR1, for penicillin regulator. Deletion of 4 bp within the identified protein binding site caused the same contrary effects on acvA and ipnA expression as observed for all of the deletion clones which lacked nucleotides -353 to -432. The PENR1 binding site thus represents a major cis-acting DNA element. The intergenic regions of the corresponding genes of the beta-lactam-producing fungi Penicillium chrysogenum and Acremonium chrysogenum also diluted the complex formed between the A. nidulans probe and PENR1 in vitro, suggesting that these beta-lactam biosynthesis genes are regulated by analogous DNA elements and proteins.
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