ArticleJournal of bacteriology1996
Mutagenesis of hydroxylamine oxidoreductase in Nitrosomonas europaea by transformation and recombination.
Article in Journal of bacteriology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed.
- Exploiting Aerobic Carboxydotrophic Bacteria for Industrial Biotechnology.Advances in biochemical engineering/biotechnology · 2022Article
- Phylogenomic analysis demonstrates a pattern of rare and long-lasting concerted evolution in prokaryotes.Communications biology · 2018Article
- Role of a Fur homolog in iron metabolism in Nitrosomonas europaea.BMC microbiology · 2011Article
- Evolution and functional characterization of the RH50 gene from the ammonia-oxidizing bacterium Nitrosomonas europaea.Journal of bacteriology · 2007Article
- Disruption of sucA, which encodes the E1 subunit of alpha-ketoglutarate dehydrogenase, affects the survival of Nitrosomonas europaea in stationary phase.Journal of bacteriology · 2006Article
- Comparative and evolutionary analysis of the bacterial homologous recombination systems.PLoS genetics · 2005Article
- Chemolithoorganotrophic growth of Nitrosomonas europaea on fructose.Journal of bacteriology · 2003Article
- Complete genome sequence of the ammonia-oxidizing bacterium and obligate chemolithoautotroph Nitrosomonas europaea.Journal of bacteriology · 2003Article
- Nitrite reductase of Nitrosomonas europaea is not essential for production of gaseous nitrogen oxides and confers tolerance to nitrite.Journal of bacteriology · 2002Article
- Transcript analysis of multiple copies of amo (encoding ammonia monooxygenase) and hao (encoding hydroxylamine oxidoreductase) in Nitrosomonas europaea.Journal of bacteriology · 2001Article
- Physical map location of the multicopy genes coding for ammonia monooxygenase and hydroxylamine oxidoreductase in the ammonia-oxidizing bacterium Nitrosomonas sp. strain ENI-11.Journal of bacteriology · 2000Article
- Isolation and characterization of two cryptic plasmids in the ammonia-oxidizing bacterium Nitrosomonas sp. strain ENI-11.Journal of bacteriology · 1999Article
- Mutagenesis and expression of amo, which codes for ammonia monooxygenase in Nitrosomonas europaea.Journal of bacteriology · 1998Article
- Cloning, nucleotide sequence, and regulatory analysis of the Nitrosomonas europaea dnaK gene.Applied and environmental microbiology · 1997Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mutagenesis of Nitrosomonas europaea was achieved by electroporation and recombination. To demonstrate this, an aminoglycoside 3'-phosphotransferase (kan) gene was specifically inserted into each of the three gene copies of hao individually. Southern hybridizations and PCR analysis showed the incorporation of the kan gene at the chosen genetic loci. The isolation of mutant strains was achieved in 7 to 14 days when the strains were grown on solid medium. The induced mutations were stable even in the absence of kanamycin-selective pressure for periods of up to 45 days in culture. The mutant strains did not show an observable phenotype different from that of the wild type when grown under the same conditions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.