ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology1995
An approach to the identification of the pathogens of bacterial meningitis by the polymerase chain reaction.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 50 citations in OpenAlex.
- Salmonella enterica serovar Virchow meningitis in a young man in Italy: a case report.Journal of medical case reports · 2014Article
- Direct detection and serogroup characterization of Neisseria meningitidis from outbreak of meningococcal meningitis in Delhi.Iranian journal of microbiology · 2010Article
- Application of atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry for rapid identification of Neisseria species.Journal of biomolecular techniques : JBT · 2008Article
- Rapid diagnosis of bacterial meningitis by real-time PCR and fluorescence in situ hybridization.Journal of clinical microbiology · 2005Article
- Loop-mediated isothermal amplification method targeting the lytA gene for detection of Streptococcus pneumoniae.Journal of clinical microbiology · 2005Article
- Laboratory detection of group B Streptococcus for prevention of perinatal disease.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2004Review
- Prospective study of use of PCR amplification and sequencing of 16S ribosomal DNA from cerebrospinal fluid for diagnosis of bacterial meningitis in a clinical setting.Journal of clinical microbiology · 2004Article
- Molecular epidemiology of group B streptococcal infections.Folia microbiologica · 2004Review
- Identification of a differentially expressed oligopeptide binding protein (OppA2) in Streptococcus uberis by representational difference analysis of cDNA.Journal of bacteriology · 2003Article
- Sensitive and specific method for rapid identification of Streptococcus pneumoniae using real-time fluorescence PCR.Journal of clinical microbiology · 2001Article
- Specific detection by PCR of Streptococcus agalactiae in milk.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2001Article
- Rapid diagnosis of bacteremia by universal amplification of 23S ribosomal DNA followed by hybridization to an oligonucleotide array.Journal of clinical microbiology · 2000Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A combination of universal and species-specific primers was used to detect and differentiate by nested polymerase chain reaction (PCR) the four species most commonly causing bacterial meningitis. Primers recognising conserved sequences in the 16S and 23S ribosomal RNA genes were employed to amplify the 16S-23S spacer region from Neisseria meningitidis, Haemophilus influenzae (type b), Streptococcus pneumoniae, and Streptococcus agalactiae (group B streptococcus). The sequence of the most abundant spacer product was determined in each case and used to deduce species-specific primers. A nested PCR using universal primers in the first round and a species-specific primer in the second were able to detect and distinguish between the four common pathogens, in the presence of human DNA. Streptococcus pneumoniae DNA was detected in the cerebrospinal fluid of a meningitis patient with negative culture and Gram-stain results.
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