ArticleMolecular biotechnology1995
One-step purification of recombinant proteins with the 6xHis tag and Ni-NTA resin.
Article in Molecular biotechnology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Light-Activated Molecular Purification (LAMP): A Photo-Responsive Affinity-Chromatography Protocol for Recombinant Protein Purification.Current protocols · 2026Article
- Characterization of Foot-and-Mouth Disease Virus Serotype O-Specific Single Domain Antibody Expressed in the pET Expression System.Indian journal of microbiology · 2023Article
- A high-throughput dual system to screen polyphosphate kinase mutants for efficient ATP regeneration in L-theanine biocatalysis.Biotechnology for biofuels and bioproducts · 2023Article
- Article
- Biosynthesis of α-Bisabolol by Farnesyl Diphosphate Synthase and α-Bisabolol Synthase and Their Related Transcription Factors inInternational journal of molecular sciences · 2023Article
- CuBiochemistry and biophysics reports · 2021Article
- Label-Free, Rapid and Facile Gold-Nanoparticles-Based Assay as a Potential Spectroscopic Tool for Trastuzumab Quantification.Nanomaterials (Basel, Switzerland) · 2021Article
- An in silico deep learning approach to multi-epitope vaccine design: a SARS-CoV-2 case study.Scientific reports · 2021Article
- Identification and Characterization of the Two-Component System HK8700-RR8701 of Kocuria rhizophila DC2201.The protein journal · 2019Article
- Biochemical characterisation of four rhamnosidases from thermophilic bacteria of the genera Thermotoga, Caldicellulosiruptor and Thermoclostridium.Scientific reports · 2019Article
- The Adsorption of Dextranase onto Mg/Fe-Layered Double Hydroxide: Insight into the Immobilization.Nanomaterials (Basel, Switzerland) · 2018Article
- The Power of Human Protective Modifiers: PLS3 and CORO1C Unravel Impaired Endocytosis in Spinal Muscular Atrophy and Rescue SMA Phenotype.American journal of human genetics · 2016Article
- Histidine biosynthesis plays a crucial role in metal homeostasis and virulence of Aspergillus fumigatus.Virulence · 2016Article
- Synthesis of poly(N-isopropylacrylamide) particles for metal affinity binding of peptides.Colloids and surfaces. B, Biointerfaces · 2014Article
- Induction of innate immune signatures following polyepitope protein-glycoprotein B-TLR4&9 agonist immunization generates multifunctional CMV-specific cellular and humoral immunity.Human vaccines & immunotherapeutics · 2014Article
- Efficient generation of rat induced pluripotent stem cells using a non-viral inducible vector.PloS one · 2013Article
- ß3 integrin modulates transforming growth factor beta induced (TGFBI) function and paclitaxel response in ovarian cancer cells.Molecular cancer · 2012Article
- Hexahistidine-tag-specific optical probes for analyses of proteins and their interactions.Analytical biochemistry · 2010Article
- An examination of the binding behavior of histidine-containing peptides with immobilized metal complexes derived from the macrocyclic ligand, 1,4,7-triazacyclononane.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2007Article
- Purification of the pyruvate dehydrogenase multienzyme complex of Zymomonas mobilis and identification and sequence analysis of the corresponding genes.Journal of bacteriology · 1998Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The 6xHis/Ni-NTA system allows rapid and efficient affinity purification of recombinant proteins from virtually any expression system. Protocols and tips for purification under both native and denaturing conditions are provided, as well as a rapid spin procedure for protein minipreps.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.