ArticleMolecular & general genetics : MGG1996
Interaction of Saccharomyces cerevisiae Pho2 with Pho4 increases the accessibility of the activation domain of Pho4.
Article in Molecular & general genetics : MGG, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Divergence in a eukaryotic transcription factor's co-TF dependence involves multiple intrinsically disordered regions.Nature communications · 2025Article
- Divergence in a Eukaryotic Transcription Factor's co-TF Dependence Involves Multiple Intrinsically Disordered Regions.bioRxiv : the preprint server for biology · 2025Article
- Plant phosphate nutrition: sensing the stress.Stress biology · 2022Review
- The Polymorphic PolyQ Tail Protein of the Mediator Complex, Med15, Regulates the Variable Response to Diverse Stresses.International journal of molecular sciences · 2020Article
- Phosphate Homeostasis - A Vital Metabolic Equilibrium Maintained Through the INPHORS Signaling Pathway.Frontiers in microbiology · 2020Review
- Evolution of reduced co-activator dependence led to target expansion of a starvation response pathway.eLife · 2017Article
- Metabolic intermediates selectively stimulate transcription factor interaction and modulate phosphate and purine pathways.Genes & development · 2009Article
- Genomic analysis of PIS1 gene expression.Eukaryotic cell · 2005Article
- Backbone dynamics of sequence specific recognition and binding by the yeast Pho4 bHLH domain probed by NMR.Protein science : a publication of the Protein Society · 2000Article
- SURVEY AND SUMMARY: Saccharomyces cerevisiae basic helix-loop-helix proteins regulate diverse biological processes.Nucleic acids research · 2000Review
- Requirements for chromatin modulation and transcription activation by the Pho4 acidic activation domain.Molecular and cellular biology · 1998Article
- Cooperative Pho2-Pho4 interactions at the PHO5 promoter are critical for binding of Pho4 to UASp1 and for efficient transactivation by Pho4 at UASp2.Molecular and cellular biology · 1998Article
- A cysteine residue in helixII of the bHLH domain is essential for homodimerization of the yeast transcription factor Pho4p.Nucleic acids research · 1998Article
- Crystal structure of PHO4 bHLH domain-DNA complex: flanking base recognition.The EMBO journal · 1997Article
- Evidence that complex formation by Bas1p and Bas2p (Pho2p) unmasks the activation function of Bas1p in an adenine-repressible step of ADE gene transcription.Molecular and cellular biology · 1997Article
- Long-range interactions at the HO promoter.Molecular and cellular biology · 1997Article
- The homeodomain protein Pho2 and the basic-helix-loop-helix protein Pho4 bind DNA cooperatively at the yeast PHO5 promoter.Nucleic acids research · 1996Article
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Authors and funding
3 authors.
Funding
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Abstract
In Saccharomyces cerevisiae, expression of acid phosphatase, encoded by the PHO5 gene, requires two positive regulatory factors, Pho4 and Pho2 (also called Bas2 or Grf10). Using GAL4-PHO4 fusions, we demonstrate that a functional interaction between these two proteins is necessary for transcriptional activation to occur. This functional interaction between Pho4 and Pho2 is independent of the presence of the negative regulatory factor, Pho80, which also interacts with Pho4. Interestingly, truncations of Pho4 missing amino acids 252-265, which encompass the basic region of the basic helix-loop-helix (bHLH) DNA binding motif, exhibit high transcriptional activation that is independent of the Pho2 molecule. Single amino acid mutations of highly conserved residues within this area all display this Pho2-independent phenotype. A region near the C-terminus of Pho2 appears to be critical for this interaction with Pho4. A model to account for the requirement for Pho2 in Pho4-dependent transcriptional activation is proposed.
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