ArticleMolecular & general genetics : MGG1996
The transcriptional activator Opaque-2 controls the expression of a cytosolic form of pyruvate orthophosphate dikinase-1 in maize endosperms.
Article in Molecular & general genetics : MGG, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37 citing papers in PubMed, 77 citations in OpenAlex.
- The Italian Research on the Molecular Characterization of Maize Kernel Development.International journal of molecular sciences · 2022Review
- Transactivation of Sus1 and Sus2 by Opaque2 is an essential supplement to sucrose synthase-mediated endosperm filling in maize.Plant biotechnology journal · 2020Article
- Over-ExpressingInternational journal of molecular sciences · 2020Article
- Pivotal role of bZIPs in amylose biosynthesis by genome survey and transcriptome analysis in wheat (Triticum aestivum L.) mutants.Scientific reports · 2018Article
- Opaque-2 Regulates a Complex Gene Network Associated with Cell Differentiation and Storage Functions of Maize Endosperm.The Plant cell · 2018Article
- Evidence for the role of transposons in the recruitment of cis-regulatory motifs during the evolution of C4 photosynthesis.BMC genomics · 2016Article
- Proteomic profiling of maize opaque endosperm mutants reveals selective accumulation of lysine-enriched proteins.Journal of experimental botany · 2016Article
- Introgression of opaque2 into Waxy Maize Causes Extensive Biochemical and Proteomic Changes in Endosperm.PloS one · 2016Article
- RNA sequencing of laser-capture microdissected compartments of the maize kernel identifies regulatory modules associated with endosperm cell differentiation.The Plant cell · 2015Article
- Genome-wide characterization of cis-acting DNA targets reveals the transcriptional regulatory framework of opaque2 in maize.The Plant cell · 2015Article
- Development and molecular characterization of genic molecular markers for grain protein and calcium content in finger millet (Eleusine coracana (L.) Gaertn.).Molecular biology reports · 2014Article
- Deletion mutagenesis identifies a haploinsufficient role for γ-zein in opaque2 endosperm modification.Plant physiology · 2014Article
- Recent advances in the study of prolamin storage protein organization and function.Frontiers in plant science · 2014Review
- Reconstruction of gene regulatory network related to photosynthesis in Arabidopsis thaliana.Frontiers in plant science · 2014Article
- Article
- OsbZIP58, a basic leucine zipper transcription factor, regulates starch biosynthesis in rice endosperm.Journal of experimental botany · 2013Article
- Kinetics and functional diversity among the five members of the NADP-malic enzyme family from Zea mays, a C4 species.Photosynthesis research · 2013Article
- Pyrophosphate-dependent fructose-6-phosphate 1-phosphotransferase induction and attenuation of Hsp gene expression during endosperm modification in quality protein maize.Plant physiology · 2012Article
- Zea mays Taxilin protein negatively regulates opaque-2 transcriptional activity by causing a change in its sub-cellular distribution.PloS one · 2012Article
- Article
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Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The maize Opaque-2 (O2) protein is a transcription factor of the basic/leucine-zipper class, involved in the regulation of endosperm proteins including the 22kDa alpha-zein storage proteins and b32 protein. In this study we have focussed our attention on the relationship between O2 and the cyPPDK1 gene, which encodes a cytoplasmic pyruvate orthophosphate dikinase (PPDK) isoform. The results of this study showed that PPDK activity is detectable in wild-type maize endosperms, while in o2 mutant endosperms, the levels of PPDK protein, mRNA and enzymatic activity are reduced, indicating that O2 is involved in the regulation of cyPPDK1 in this tissue. By employing transient expression experiments in tobacco mesophyll protoplasts, we have demonstrated that the O2 protein can activate expression of a chloramphenicol acetyl transferase reporter gene placed under the control of the cyPPDK1 promoter. An in vitro binding assay and DNaseI footprint analysis demonstrated that a specific sequence in the cyPPDK1 promoter can be recognized and protected by maize O2 protein. The regulation by the O2 locus of cyPPDK1 reported here, and control of alpha-zein synthesis by O2 suggest that the O2 protein may play a more general role in maize endosperm development than previously thought.
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