ArticleMolecular & general genetics : MGG1996
Characterization and subcellular localization of a small GTP-binding protein (Ara-4) from Arabidopsis: conditional expression under control of the promoter of the gene for heat-shock protein HSP81-1.
Article in Molecular & general genetics : MGG, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 62 citations in OpenAlex.
- Plant RABs: Role in Development and in Abiotic and Biotic Stress Responses.Current genomics · 2021Review
- Genome-wide identification and comparative analysis of alternative splicing across four legume species.Planta · 2019Article
- The Specification of Geometric Edges by a Plant Rab GTPase Is an Essential Cell-Patterning Principle During Organogenesis in Arabidopsis.Developmental cell · 2016Article
- BEX5/RabA1b regulates trans-Golgi network-to-plasma membrane protein trafficking in Arabidopsis.The Plant cell · 2012Article
- Vacuolar transport in tobacco leaf epidermis cells involves a single route for soluble cargo and multiple routes for membrane cargo.The Plant cell · 2011Article
- Rab-A2 and Rab-A3 GTPases define a trans-golgi endosomal membrane domain in Arabidopsis that contributes substantially to the cell plate.The Plant cell · 2008Article
- The Arabidopsis AAA ATPase SKD1 is involved in multivesicular endosome function and interacts with its positive regulator LYST-INTERACTING PROTEIN5.The Plant cell · 2007Article
- Rab11 GTPase-regulated membrane trafficking is crucial for tip-focused pollen tube growth in tobacco.The Plant cell · 2005Article
- Identification and characterization of AtCASP, a plant transmembrane Golgi matrix protein.Plant molecular biology · 2005Article
- A Rab-related small GTP binding protein is predominantly expressed in root nodules of Medicago sativa.Molecular genetics and genomics : MGG · 2004Article
- The Arabidopsis Rab GTPase RabA4b localizes to the tips of growing root hair cells.The Plant cell · 2004Article
- Rab2 GTPase regulates vesicle trafficking between the endoplasmic reticulum and the Golgi bodies and is important to pollen tube growth.The Plant cell · 2002Article
- The ANGUSTIFOLIA gene of Arabidopsis, a plant CtBP gene, regulates leaf-cell expansion, the arrangement of cortical microtubules in leaf cells and expression of a gene involved in cell-wall formation.The EMBO journal · 2002Article
- Ara6, a plant-unique novel type Rab GTPase, functions in the endocytic pathway of Arabidopsis thaliana.The EMBO journal · 2001Article
- A cyclin-dependent kinase-activating kinase regulates differentiation of root initial cells in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- A rab1 GTPase is required for transport between the endoplasmic reticulum and golgi apparatus and for normal golgi movement in plants.The Plant cell · 2000Article
- Nucleoside diphosphate kinase required for coleoptile elongation in rice.Plant physiology · 2000Article
- Changes in the shapes of leaves and flowers upon overexpression of cytochrome P450 in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Exocytosis in plants.Plant molecular biology · 1998Review
- A putative vacuolar cargo receptor partially colocalizes with AtPEP12p on a prevacuolar compartment in Arabidopsis roots.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Small GTP-binding proteins belonging to the rab/YPT family play key roles at various steps in intracellular transport pathways in yeast and mammalian cells. Many members of rab/YPT family have been isolated from plants to date. However, detailed information about the localization and function of the gene products remains limited, even though intracellular transport is likely to be involved in important phenomena such as cell elongation, transport of storage proteins, determination and maintenance of cell polarity and intercellular signal transduction. We have attempted to establish transgenic Arabidopsis plants that overexpress ARA-4, a rab/YPT homologue in order to analyze the function and the localization of the gene product. For overexpression and also for regulation of the expression of this gene, the promoter of the gene for HSP81-1 was employed to drive the transcription of ARA-4 in transgenic plants. The response of the introduced genes to heat shock was analyzed. Upon heat-shock treatment, the ARA-4 gene was efficiently transcribed and translated. The induction of ARA-4 by heat shock was transient, and at least two distinct forms of this protein were found in membrane and cytosolic fractions from transgenic plants. Prolonged incubation after heat shock reduced the amount of the cytosolic form of the induced protein, and the cytosolic form of the protein thus probably represents the unprocessed precursor. Using transgenic plants, we determined the subcellular localization of the product of ARA-4. The protein was predominantly localized on Golgi-derived vesicles, Golgi cisternae and the trans-Golgi network.
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