ArticleThe Journal of clinical investigation1996
Rat renal arcade segment expresses vasopressin-regulated water channel and vasopressin V2 receptor.
Article in The Journal of clinical investigation, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 74 citations in OpenAlex.
- Developmental origins of disease - Effects of iron deficiency in the rat developing kidney and beyond.Pediatric nephrology (Berlin, Germany) · 2026Review
- Factor VIII originates primarily from anatomically distinct subsets of liver sinusoidal endothelial cells.Blood advances · 2026Article
- Non-coding RNAs and Aquaporin 4: Their Role in the Pathogenesis of Neurological Disorders.Neurochemical research · 2024Review
- Physical Processes in Polymeric Filters Used for Dialysis.Polymers · 2019Review
- Plasma copeptin as a predictor of kidney disease.Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association · 2019Article
- From Molecules to Mechanisms: Functional Proteomics and Its Application to Renal Tubule Physiology.Physiological reviews · 2018Review
- Tolvaptan regulates aquaporin-2 and fecal water in cirrhotic rats with ascites.World journal of gastroenterology · 2016Article
- Understanding the role of maternal diet on kidney development; an opportunity to improve cardiovascular and renal health for future generations.Nutrients · 2015Review
- Distal convoluted tubule.Comprehensive Physiology · 2015Review
- Physiological roles of aquaporin-4 in brain.Physiological reviews · 2013Review
- Immunofluorescent localization of the Rab-GAP protein TBC1D4 (AS160) in mouse kidney.Histochemistry and cell biology · 2012Article
- The role of proximal nephron in cyclophosphamide-induced water retention: preliminary data.Electrolyte & blood pressure : E & BP · 2011Article
- Sodium and potassium balance depends on αENaC expression in connecting tubule.Journal of the American Society of Nephrology : JASN · 2010Article
- Expression and immunolocalization of ERG1 potassium channels in the rat kidney.Histochemistry and cell biology · 2010Article
- Role of pendrin in iodide balance: going with the flow.American journal of physiology. Renal physiology · 2009Article
- Severe urinary concentrating defect in renal collecting duct-selective AQP2 conditional-knockout mice.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Article
- Annexin A4 reduces water and proton permeability of model membranes but does not alter aquaporin 2-mediated water transport in isolated endosomes.The Journal of general physiology · 2003Article
- Role of renal aquaporins in escape from vasopressin-induced antidiuresis in rat.The Journal of clinical investigation · 1997Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 1 country.
Funding
Abstract
The arcades are long, branched renal tubules which connect deep and mid-cortical nephrons to cortical collecting ducts in the renal cortex. Because they are inaccessible by standard physiological techniques, their functions are poorly understood. In this paper, we demonstrate that the arcades are a site of expression of two proteins, aquaporin-2 (the vasopressin-regulated water channel) and the V2 vasopressin receptor, that are important to regulated water transport in the kidney. Using a peptide-derived polyclonal antibody to aquaporin-2, quantitative ELISA in microdissected segments showed that aquaporin-2 is highly expressed in arcades and that the expression is increased in response to restriction of fluid intake. Immunocytochemistry revealed abundant aquaporin-2 labeling of structures in the cortical labyrinth in a pattern similar to that of the Na(+)-Ca2+ exchanger and kallikrein, marker proteins expressed in arcades but not in cortical collecting ducts. RT-PCR experiments demonstrated substantial aquaporin-2 and V2 receptor mRNA in microdissected arcades. In situ hybridization, using 35S-labeled antisense cRNA probes for the V2 receptor demonstrated strong labeling of both arcades and cortical collecting ducts. Thus, these results indicate that the arcades contain the specific proteins associated with vasopressin-regulated water transport, and may be a heretofore unrecognized site of free water absorption.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.