ArticleThe Journal of clinical investigation1995
Glucose transport in cultured human skeletal muscle cells. Regulation by insulin and glucose in nondiabetic and non-insulin-dependent diabetes mellitus subjects.
Article in The Journal of clinical investigation, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT04334343 (Investigating the Effects of Aerobic and Resistance Training in Vivo on Skeletal Muscle Metabolism in Vitro in Primary Human Muscle Cells), which is not on this map. Cited by 44 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Investigating the Effects of Aerobic and Resistance Training in Vivo on Skeletal Muscle Metabolism in Vitro in Primary Human Muscle Cells (MoTrMyo)
Who cites it
44 citing papers in PubMed, 125 citations in OpenAlex.
- Circulating Biomarkers as a Window into the Relationship between Sarcopenia and Diabetes: Implications for Effective Management.Current diabetes reviews · 2026Review
- Insights into human muscle biology from human primary skeletal muscle cell culture.Journal of muscle research and cell motility · 2025Review
- Insulin Resistance, Obesity, and Lipotoxicity.Advances in experimental medicine and biology · 2024Review
- Activation of PDGF Signaling in the Adult Muscle Stem Cell Niche in Patients With Type 2 Diabetes Mellitus.The Journal of clinical endocrinology and metabolism · 2023Article
- Review
- p300 or CBP is required for insulin-stimulated glucose uptake in skeletal muscle and adipocytes.JCI insight · 2022Article
- Exogenous detection ofFrontiers in physiology · 2022Article
- Human and mouse muscle transcriptomic analyses identify insulin receptor mRNA downregulation in hyperinsulinemia-associated insulin resistance.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Article
- Redox changes in obesity, metabolic syndrome, and diabetes.Redox biology · 2021Review
- Glucose Uptake and Insulin Response in Tissue-engineered Human Skeletal Muscle.Tissue engineering and regenerative medicine · 2020Article
- Engineered skeletal muscles for disease modeling and drug discovery.Biomaterials · 2019Review
- Athletes feature greater rates of muscle glucose transport and glycogen synthesis during lipid infusion.JCI insight · 2019Article
- The effect of type 2 diabetes mellitus and obesity on muscle progenitor cell function.Stem cell research & therapy · 2019Review
- Myokine Regulation of Insulin Secretion: Impact of Inflammation and Type 2 Diabetes.Frontiers in physiology · 2019Article
- Development and application of human skeletal muscle microphysiological systems.Lab on a chip · 2018Review
- In vitro contraction protects against palmitate-induced insulin resistance in C2C12 myotubes.American journal of physiology. Cell physiology · 2017Article
- Mitochondrial HDiabetologia · 2017Article
- Altered Myokine Secretion Is an Intrinsic Property of Skeletal Muscle in Type 2 Diabetes.PloS one · 2016Article
- Modest hyperglycemia prevents interstitial dispersion of insulin in skeletal muscle.Metabolism: clinical and experimental · 2015Article
- Antipsychotics inhibit glucose transport: Determination of olanzapine binding site in Staphylococcus epidermidis glucose/H(+) symporter.FEBS open bio · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
A primary human skeletal muscle culture (HSMC) system, which retains cellular integrity and insulin responsiveness for glucose transport was employed to evaluate glucose transport regulation. As previously reported, cells cultured from non-insulin-dependent diabetic (NIDDM) subjects displayed significant reductions in both basal and acute insulin-stimulated transport compared to nondiabetic controls (NC). Fusion/differentiation of NC and NIDDM HSMC in elevated media insulin (from 22 pM to 30 microM) resulted in increased basal transport activities but reduced insulin-stimulated transport, so that cells were no longer insulin responsive. After fusion under hyperinsulinemic conditions, GLUT1 protein expression was elevated in both groups while GLUT4 protein level was unaltered. Fusion of HSMC under hyperglycemic conditions (10 and 20 mM) decreased glucose transport in NC cells only when combined with hyperinsulinemia. Hyperglycemia alone down-regulated transport in HSMC of NIDDM, while the combination of hyperglycemia and hyperinsulinemia had greater effects. In summary: (a) insulin resistance of glucose transport can be induced in HSMC of both NC and NIDDM by hyperinsulinemia and is accompanied by unaltered GLUT4 but increased GLUT1 levels; and (b) HSMC from NIDDM subjects demonstrate an increased sensitivity to impairment of glucose transport by hyperglycemia. These results indicate that insulin resistance in skeletal muscle can be acquired in NC and NIDDM from hyperinsulinemia alone but that NIDDM is uniquely sensitive to the additional influence of hyperglycemia.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.