ArticleThe Plant cell1996
A null mutation in the first enzyme of flavonoid biosynthesis does not affect male fertility in Arabidopsis.
Article in The Plant cell, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 88 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
88 citing papers in PubMed, 206 citations in OpenAlex.
- A case study of evolutionary variability and determinism among type III polyketide synthases.Nature communications · 2026Article
- Antioxidant properties of dihydroxy B-ring flavonoids modulate circadian amplitude inLife science alliance · 2025Article
- An R2R3 MYB transcription factor GhMYB5: regulator of CHS expression and proanthocyanin synthesis in brown cotton (Gossypium hirsutum L.).BMC genomics · 2025Article
- Identification and Expression Analysis of Chalcone Synthase Gene Family in Tartary Buckwheat.Genes · 2025Article
- Megagametophyte maturation dynamics and flavonol changes duringFrontiers in plant science · 2025Article
- Transcriptome and Metabolome Analyses Reveal a Complex Stigma Microenvironment for Pollen Tube Growth in Tobacco.International journal of molecular sciences · 2024Article
- Flavonoids and anthocyanins in seagrasses: implications for climate change adaptation and resilience.Frontiers in plant science · 2024Review
- Characterization and Functional Analysis ofInternational journal of molecular sciences · 2023Article
- Homeostasis of flavonoids and triterpenoids most likely modulates starch metabolism for pollen tube penetration in rice.Plant biotechnology journal · 2023Article
- Transcriptome Analysis Reveals Changes in Whole Gene Expression, Biological Process, and Molecular Functions Induced by Nickel in Jack Pine (Plants (Basel, Switzerland) · 2023Article
- Brachypodium BdCHS is a homolog of Arabidopsis AtCHS involved in the synthesis of flavonoids and lateral root development.Protoplasma · 2023Article
- Flavonols modulate plant development, signaling, and stress responses.Current opinion in plant biology · 2023Review
- Phenylalanine Ammonia LyasePlants (Basel, Switzerland) · 2022Article
- Reactive oxygen species function as signaling molecules in controlling plant development and hormonal responses.Current opinion in plant biology · 2022Review
- Mutations that alter Arabidopsis flavonoid metabolism affect the circadian clock.The Plant journal : for cell and molecular biology · 2022Article
- Combined analysis of transcriptome and metabolome reveals that sugar, lipid, and phenylpropane metabolism are essential for male fertility in temperature-induced male sterile rice.Frontiers in plant science · 2022Article
- Comparative Metabolic Analysis Reveals a Metabolic Switch in Mature, Hydrated, and Germinated Pollen inFrontiers in plant science · 2022Article
- Involvement of the R2R3-MYB transcription factor MYB21 and its homologs in regulating flavonol accumulation in Arabidopsis stamen.Journal of experimental botany · 2021Article
- Metabolic source isotopic pair labeling and genome-wide association are complementary tools for the identification of metabolite-gene associations in plants.The Plant cell · 2021Article
- Insights into the Role of Transcriptional Gene Silencing in Response to Herbicide-Treatments inInternational journal of molecular sciences · 2021Article
28 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Flavonoids are a major class of secondary metabolites that serves a multitude of functions in higher plants, including a recently discovered role in male fertility. Surprisingly, Arabidopsis plants deficient in flavonoid biosynthesis appear to be fully fertile. Using RNA gel blot analysis and polymerase chain reaction-based assays, we have shown that a mutation at the 3' splice acceptor site in the Arabidopsis chalcone synthase gene completely disrupts synthesis of the active form of the enzyme. We also confirmed that this enzyme, which catalyzes the first step of flavonoid biosynthesis, is encoded by a single-copy gene. HPLC analysis of whole flowers and stamens was used to show that plants homozygous for the splice site mutation are completely devoid of flavonoids. This work provides compelling evidence that despite the high levels of these compounds in the pollen of most plant species, flavonoids are not universally required for fertility. The role of flavonoids in plant reproduction may therefore offer an example of convergent functional evolution in secondary metabolism.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.