ArticleThe Plant cell1996
Transcription factor veracity: is GBF3 responsible for ABA-regulated expression of Arabidopsis Adh?
Article in The Plant cell, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 92 citations in OpenAlex.
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- Organ-level gene-regulatory networks inferred from transcriptomic data reveal context-specific regulation and highlight novel regulators of ripening and ABA-mediated responses in tomato.Plant communications · 2025Article
- Plasma-treated water applied as a foliar spray promotes root growth in barley.BMC plant biology · 2025Article
- Developmental cues are encoded by the combinatorial phosphorylation of Arabidopsis RETINOBLASTOMA-RELATED protein RBR1.The EMBO journal · 2024Article
- Integrating gene expression analysis and ecophysiological responses to water deficit in leaves of tomato plants.Scientific reports · 2024Article
- The dimorphic diaspore model Aethionema arabicum (Brassicaceae): Distinct molecular and morphological control of responses to parental and germination temperatures.The Plant cell · 2024Article
- Kiwifruit MYBS1-like and GBF3 transcription factors influence l-ascorbic acid biosynthesis by activating transcription of GDP-L-galactose phosphorylase 3.The New phytologist · 2022Article
- Network Inference of Transcriptional Regulation in Germinating Low Phytic Acid Soybean Seeds.Frontiers in plant science · 2021Article
- Transgenic Breeding Approaches for Improving Abiotic Stress Tolerance: Recent Progress and Future Perspectives.International journal of molecular sciences · 2020Review
- Time-Course Transcriptome Study Reveals Mode of bZIP Transcription Factors on Light Exposure inInternational journal of molecular sciences · 2020Article
- Dynamics of ADH and related genes responsible for the transformation of CFood science & nutrition · 2020Article
- Overexpression of a SBP-Box Gene (VpSBP16) from Chinese Wild Vitis Species in Arabidopsis Improves Salinity and Drought Stress Tolerance.International journal of molecular sciences · 2018Article
- GBF3 transcription factor imparts drought tolerance in Arabidopsis thaliana.Scientific reports · 2017Article
- A transcription factor hierarchy defines an environmental stress response network.Science (New York, N.Y.) · 2016Article
- Stress-MediatedFrontiers in plant science · 2016Review
- Redundant ERF-VII Transcription Factors Bind to an Evolutionarily Conserved cis-Motif to Regulate Hypoxia-Responsive Gene Expression in Arabidopsis.The Plant cell · 2016Article
- Large scale transcriptome analysis reveals interplay between development of forest trees and a beneficial mycorrhiza helper bacterium.BMC genomics · 2015Article
- Arabidopsis ensemble reverse-engineered gene regulatory network discloses interconnected transcription factors in oxidative stress.The Plant cell · 2014Article
- Microarray analysis of transcriptional responses to abscisic acid and salt stress in Arabidopsis thaliana.International journal of molecular sciences · 2013Article
- Microarray analysis of the moss Physcomitrella patens reveals evolutionarily conserved transcriptional regulation of salt stress and abscisic acid signalling.Plant molecular biology · 2010Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Assignment of particular transcription factors to specific roles in promoter elements can be problematic, especially in systems such as the G-box, where multiple factors of overlapping specificity exist. In the Arabidopsis alcohol dehydrogenase (Adh) promoter, the G-box regulates expression in response to cold and dehydration, presumably through the action of abscisic acid (ABA), and is bound by a nuclear protein complex in vivo during expression in cell cultures. In this report, we test the conventional wisdom of biochemical approaches used to identify DNA binding proteins and assess their specific interactions by using the G-box and a nearby half G-box element of the Arabidopsis Adh promoter as a model system. Typical in vitro assays demonstrated specific interaction of G-box factor 3 (GBF3) with both the G-box and the half G-box element. Dimethyl sulfate footprint analysis confirmed that the in vitro binding signature of GBF3 essentially matches the footprint signature detected in vivo at the G-box. Because RNA gel blot data indicated that GBF3 is itself induced by ABA, we might have concluded that GBF3 is indeed the GBF responsible in cell cultures for binding to the Adh G-box and is therefore responsible for ABA-regulated expression of Adh. Potential limitations of this conclusion are exposed by the fact that other GBFs bind the G-box with the same signature as GBF3, and subtle differences between in vivo and in vitro footprint signatures indicate that factors other than or in addition to GBF3 interact with the half G-box element.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.