ArticleMammalian genome : official journal of the International Mammalian Genome Society1996
Evaluation and characterization of a porcine small intestine cDNA library: analysis of 839 clones.
Article in Mammalian genome : official journal of the International Mammalian Genome Society, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 68 citations in OpenAlex.
- Article
- A study of alternative splicing in the pig.BMC research notes · 2010Article
- A pig multi-tissue normalised cDNA library: large-scale sequencing, cluster analysis and 9K micro-array resource generation.BMC genomics · 2008Article
- Advances in swine transcriptomics.International journal of biological sciences · 2007Review
- Generation and analysis of large-scale expressed sequence tags (ESTs) from a full-length enriched cDNA library of porcine backfat tissue.BMC genomics · 2006Article
- Partial amino acid sequence and mRNA analysis of cytosolic pyridoxine-beta-D-glucoside hydrolase from porcine intestinal mucosa: proposed derivation from the lactase-phlorizin hydrolase gene.The Biochemical journal · 2004Article
- EST-based gene discovery in pig: virtual expression patterns and comparative mapping to human.Mammalian genome : official journal of the International Mammalian Genome Society · 2003Article
- Comparative mapping in the pig: localization of 214 expressed sequence tags.Mammalian genome : official journal of the International Mammalian Genome Society · 2003Article
- Advances in pig genomics and functional gene discovery.Comparative and functional genomics · 2003Article
- The molecular basis for hereditary porcine membranoproliferative glomerulonephritis type II: point mutations in the factor H coding sequence block protein secretion.The American journal of pathology · 2002Article
- Purification and characterization of a glutathione S-transferase Omega in pig: evidence for two distinct organ-specific transcripts.The Biochemical journal · 2001Article
- Chromosomal assignments of 19 porcine cDNA sequences by FISH.Mammalian genome : official journal of the International Mammalian Genome Society · 1998Article
- Improvement of the porcine transcription map: localization of 33 genes, of which 24 are orthologous.Mammalian genome : official journal of the International Mammalian Genome Society · 1998Article
- FISH mapping of seven cDNA sequences in the pig.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 1997Article
- Expansion of the pig comparative map by expressed sequence tags (EST) mapping.Mammalian genome : official journal of the International Mammalian Genome Society · 1997Article
- The cytogenetic map of the domestic pig.Mammalian genome : official journal of the International Mammalian Genome Society · 1997Article
- Mapping of 22 expressed sequence tags isolated from a porcine small intestine cDNA library.Mammalian genome : official journal of the International Mammalian Genome Society · 1997Article
- A first catalog of genes involved in pig ovarian follicular differentiation.Mammalian genome : official journal of the International Mammalian Genome Society · 1997Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A porcine small intestine directionally cloned cDNA library was constructed in the vector lambda Zap II. Clones were hybridized with total labeled cDNA such that putative high-copy number transcripts could be differentiated from middle- and low-copy number transcripts prior to selection and characterization by DNA sequencing. More than 2000 non-hybridizing and 242 hybridizing clones were collected. In total, 839 clones were sequenced from the 3' end of the cDNA, and after inter-clone comparison, the unique clones were sequenced from the 5' end of the cDNA. The 5' data were used to query the sequence in databases and resulted in the identification of 630 different gene transcripts, of which 604 are new porcine genes. The identity of 361 transcripts could be identified from sequence comparison studies. The validity of this semi-random selection approach was verified by the identification of a large number of unique transcripts.
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