ArticleThe EMBO journal1996
Evidence for the presence of a small U5-like RNA in active trans-spliceosomes of Trypanosoma brucei.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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Who cites it
21 citing papers in PubMed, 56 citations in OpenAlex.
- Structural basis of step II spliced leader RNA trans-splicing in trypanosomatid parasites.Nature communications · 2026Article
- A comparative genome-wide study of ncRNAs in trypanosomatids.BMC genomics · 2010Article
- The pre-mRNA splicing machinery of trypanosomes: complex or simplified?Eukaryotic cell · 2010Review
- Special Sm core complex functions in assembly of the U2 small nuclear ribonucleoprotein of Trypanosoma brucei.Eukaryotic cell · 2009Article
- Trypanosome spliced-leader-associated RNA (SLA1) localization and implications for spliced-leader RNA biogenesis.Eukaryotic cell · 2009Article
- Identification of novel snRNA-specific Sm proteins that bind selectively to U2 and U4 snRNAs in Trypanosoma brucei.RNA (New York, N.Y.) · 2007Article
- Sm core variation in spliceosomal small nuclear ribonucleoproteins from Trypanosoma brucei.The EMBO journal · 2006Article
- trans and cis splicing in trypanosomatids: mechanism, factors, and regulation.Eukaryotic cell · 2003Review
- The spliced leader-associated RNA is a trypanosome-specific sn(o) RNA that has the potential to guide pseudouridine formation on the SL RNA.RNA (New York, N.Y.) · 2002Article
- Unconventional rules of small nuclear RNA transcription and cap modification in trypanosomatids.Gene expression · 2002Review
- Determinants for cap trimethylation of the U2 small nuclear RNA are not conserved between Trypanosoma brucei and higher eukaryotic organisms.Nucleic acids research · 2000Article
- trans splicing of polycistronic Caenorhabditis elegans pre-mRNAs: analysis of the SL2 RNA.Molecular and cellular biology · 2000Article
- The spliceosomal snRNP core complex of Trypanosoma brucei: cloning and functional analysis reveals seven Sm protein constituents.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- U1 small nuclear RNA and spliceosomal introns in Euglena gracilis.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Three small nucleolar RNAs identified from the spliced leader-associated RNA locus in kinetoplastid protozoans.Molecular and cellular biology · 1998Article
- Exonic sequences in the 5' untranslated region of alpha-tubulin mRNA modulate trans splicing in Trypanosoma brucei.Molecular and cellular biology · 1998Article
- Structure-function analysis of the trypanosomatid spliced leader RNA.Nucleic acids research · 1998Article
- Characterization of a novel trypanosomatid small nucleolar RNA.Nucleic acids research · 1998Article
- The U5 RNA of trypanosomes deviates from the canonical U5 RNA: the Leptomonas collosoma U5 RNA and its coding gene.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Small RNA database.Nucleic acids research · 1997Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The existence of the Trypanosoma brucei 5' splice site on a small RNA of uniform sequence (the spliced leader or SL RNA) has allowed us to characterize the RNAs with which it interacts in vivo by psoralen crosslinking treatment. Analysis of the most abundant crosslinks formed by the SL RNA allowed us previously to identify the spliced leader-associated (SLA) RNA. The role of this RNA in trans-splicing, as well as the possible existence of an analogous RNA interaction in cis-splicing, is unknown. We show here that the 5' splice site region of the SL RNA is also crosslinked in vivo to a second small RNA. Although it is very small and lacks a 5' trimethylguanosine (TMG) cap, the SLA2RNA possesses counterparts of the conserved U5 snRNA stem-loop 1 and internal loop 1 sequence elements, as well as a potential trypanosome snRNA core protein binding site; these combined features meet the phylogenetic definition of U5 snRNA. Like U5, the SLA2 RNA forms an RNP complex with the U4 and U6 RNAs, and interacts with the 5' splice site region via its putative loop 1 sequence. In a final analogy with U5, the SLA2 RNA is found crosslinked to a molecule identical to the free 5' exon splicing intermediate. These data present a compelling case for the SLA2 RNA not only as an active trans-spliceosomal component, but also for its identification as the trypanosome U5 structural homolog. The presence of a U5-like RNA in this ancient eukaryote establishes the universality of the spliceosomal RNA core components.
Indexed as
Identifiers
8670906PMC452122W2266792252What OpenQuestion holds
Registered trials
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