ArticleThe EMBO journal1996
Quantitation of putative activator-target affinities predicts transcriptional activating potentials.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.
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Who cites it
81 citing papers in PubMed, 191 citations in OpenAlex.
- Article
- Systems for Targeted Silencing of Gene Expression and Their Application in Plants and Animals.International journal of molecular sciences · 2024Review
- Transcription factor clusters enable target search but do not contribute to target gene activation.Nucleic acids research · 2023Article
- Zinc cluster transcription factors frequently activate target genes using a non-canonical half-site binding mode.Nucleic acids research · 2023Article
- Comparison of GAL80ts and Tet-off GAL80 transgenes.microPublication biology · 2023Article
- Transcriptional repressor Gal80 recruits corepressor complex Cyc8-Tup1 to structural genes of the Saccharomyces cerevisiae GAL regulon.Current genetics · 2022Article
- Variation in the modality of a yeast signaling pathway is mediated by a single regulator.eLife · 2021Article
- Mediator subunit Med15 dictates the conserved "fuzzy" binding mechanism of yeast transcription activators Gal4 and Gcn4.Nature communications · 2021Article
- Quantitative analysis of transcription factor binding and expression using calling cards reporter arrays.Nucleic acids research · 2020Article
- A Tet/Q Hybrid System for Robust and Versatile Control of Transgene Expression in C. elegans.iScience · 2019Article
- The 9aaTAD Is Exclusive Activation Domain in Gal4.PloS one · 2017Article
- Regulating the UAS/GAL4 system in adultPeerJ · 2017Article
- A Modified Reverse One-Hybrid Screen Identifies Transcriptional Activation Domains in PHYTOCHROME-INTERACTING FACTOR 3.Frontiers in plant science · 2016Article
- Nucleosome distortion as a possible mechanism of transcription activation domain function.Epigenetics & chromatin · 2016Review
- The 9aaTAD Transactivation Domains: From Gal4 to p53.PloS one · 2016Article
- New insights for Drosophila GAGA factor in larvae.Royal Society open science · 2015Article
- A targeted gene expression system using the tryptophan repressor in zebrafish shows no silencing in subsequent generations.Development (Cambridge, England) · 2014Article
- The chemistry of regulation of genes and other things.The Journal of biological chemistry · 2014Review
- Inducible, tightly regulated and growth condition-independent transcription factor in Saccharomyces cerevisiae.Nucleic acids research · 2014Article
- Interplay of a ligand sensor and an enzyme in controlling expression of the Saccharomyces cerevisiae GAL genes.Eukaryotic cell · 2012Article
21 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We quantitate the 'activating potentials' of deletion and point mutation variants of a 42 amino acid yeast transcriptional activating region excised from the yeast activator GAL4 and, using surface plasmon resonance, we measure the relative affinities of these molecules for a variety of proteins, including plausible target proteins as well as GAL80, a specific inhibitor of GAL4. We find a remarkable correlation between the relative activating potentials of the derivatives and their relative affinities for yeast TBP and for yeast TFIIB; other tested proteins interacted significantly more weakly, if at all. These results provide an especially strong argument that TBP and TFIIB are activating region targets. We also show, using one set of yeast activating region mutants, that activator-target interactions are strongly correlated with the length of the activating region, that the effect of point mutants is highly dependent on the length of the activating region mutated and that, unlike interactions with TBP and TFIIB, interaction with the specific inhibitor GAL80 is destroyed by deletion of certain critical residues in the C-terminal half of the 42 amino acid activating region.
Indexed as
Identifiers
8670900PMC452115W45968920What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.