ArticleThe EMBO journal1996
Replacement of two non-adjacent amino acids in the S.cerevisiae bi2 intron-encoded RNA maturase is sufficient to gain a homing-endonuclease activity.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 51 citations in OpenAlex.
- Mitochondrial DNA duplication, recombination, and introgression during interspecific hybridization.Scientific reports · 2021Article
- The diversity of mtDNA rns introns among strains of Ophiostoma piliferum, Ophiostoma pluriannulatum and related species.SpringerPlus · 2016Article
- The transcriptome of Candida albicans mitochondria and the evolution of organellar transcription units in yeasts.BMC genomics · 2015Article
- Homing endonucleases: from genetic anomalies to programmable genomic clippers.Methods in molecular biology (Clifton, N.J.) · 2014Review
- Structural, functional and evolutionary relationships between homing endonucleases and proteins from their host organisms.Nucleic acids research · 2012Review
- Homing endonucleases: from microbial genetic invaders to reagents for targeted DNA modification.Structure (London, England : 1993) · 2011Review
- Article
- Evolutionary origin of a plant mitochondrial group II intron from a reverse transcriptase/maturase-encoding ancestor.Journal of plant research · 2006Article
- Intragenic suppressors that restore the activity of the maturase encoded by the second intron of the Saccharomyces cerevisiae cyt b gene.Current genetics · 2004Article
- Genetic conservation versus variability in mitochondria: the architecture of the mitochondrial genome in the petite-negative yeast Schizosaccharomyces pombe.Current genetics · 2003Review
- The Rieske FeS protein encoded and synthesized within mitochondria complements a deficiency in the nuclear gene.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Recruitment of intron-encoded and co-opted proteins in splicing of the bI3 group I intron RNA.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Homing endonucleases: structural and functional insight into the catalysts of intron/intein mobility.Nucleic acids research · 2001Review
- Purification and characterization of the DNA cleavage and recognition site of I-ScaI mitochondrial group I intron encoded endonuclease produced in Escherichia coli.Nucleic acids research · 2000Article
- The protein splicing domain of the homing endonuclease PI-sceI is responsible for specific DNA binding.Nucleic acids research · 1998Article
- Homing endonucleases: keeping the house in order.Nucleic acids research · 1997Review
- A protein encoded by a group I intron in Aspergillus nidulans directly assists RNA splicing and is a DNA endonuclease.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two homologous group I introns, the second intron of the cyt b gene, from related Saccharomyces species differ in their mobility. The S.capensis intron is mobile and encodes the I-ScaI endonuclease promoting intron homing, whilst the homologous S.cerevisiae intron is not mobile, but functions as an RNA maturase promoting splicing. These two intron-encoded proteins differ by only four amino acid substitutions. Taking advantage of the remarkable similarity of the two intron open reading frames and using biolistic transformation of mitochondria, we show that the replacement of only two non-adjacent residues in the S.cerevisiae maturase carboxy-terminal sequence is sufficient to induce a homing-endonuclease activity without losing the splicing function. Also, we demonstrate that these two activities reside in the S.capensis bi2-encoded protein which functions in both splicing and intron mobility in the wild-type cells. These results provide new insight into our understanding of the activity and the evolution of group I intron-encoded proteins.
Indexed as
Identifiers
8670880PMC452048W160333799What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.