ArticleThe EMBO journal1996
The beta-globin locus control region enhances transcription of but does not confer position-independent expression onto the lacZ gene in transgenic mice.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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Who cites it
18 citing papers in PubMed, 66 citations in OpenAlex.
- In search of lost time: Enhancers as modulators of timing in lymphocyte development and differentiation.Immunological reviews · 2021Review
- Efficient and Reproducible Multigene Expression after Single-Step Transfection Using Improved BAC Transgenesis and Engineering Toolkit.ACS synthetic biology · 2020Article
- Distinct roles for Ste20-like kinase SLK in muscle function and regeneration.Skeletal muscle · 2013Article
- BAC TG-EMBED: one-step method for high-level, copy-number-dependent, position-independent transgene expression.Nucleic acids research · 2010Article
- Bin1 SRC homology 3 domain acts as a scaffold for myofiber sarcomere assembly.The Journal of biological chemistry · 2009Article
- Chromatin context dominates estrogen regulation of pS2 gene expression.Experimental cell research · 2008Article
- The SCL +40 enhancer targets the midbrain together with primitive and definitive hematopoiesis and is regulated by SCL and GATA proteins.Molecular and cellular biology · 2007Article
- Artificial chromosome-based transgenes in the study of genome function.Mammalian genome : official journal of the International Mammalian Genome Society · 2006Review
- Transcriptional regulation of the SCL locus: identification of an enhancer that targets the primitive erythroid lineage in vivo.Molecular and cellular biology · 2005Article
- Functional dissection of the mouse tyrosinase locus control region identifies a new putative boundary activity.Nucleic acids research · 2003Article
- Spatial organization of gene expression: the active chromatin hub.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2003Review
- Article
- Burkitt lymphoma in the mouse.The Journal of experimental medicine · 2000Article
- Pre-selection of integration sites imparts repeatable transgene expression.Nucleic acids research · 2000Article
- Position effects in mice carrying a lacZ transgene in cis with the beta-globin LCR can be explained by a graded model.Nucleic acids research · 1997Article
- The developmental activation of the chicken lysozyme locus in transgenic mice requires the interaction of a subset of enhancer elements with the promoter.Nucleic acids research · 1997Article
- Transcriptional regulation in endoderm development: characterization of an enhancer controlling Hnf3g expression by transgenesis and targeted mutagenesis.The EMBO journal · 1997Article
- Mammalian cDNA and prokaryotic reporter sequences silence adjacent transgenes in transgenic mice.Nucleic acids research · 1997Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The beta-globin locus control region (LCR) confers high levels of position-independent, copy number-dependent expression onto globin transgenes. Here > 40 independent transgenic mouse lines and founders that carried the LCR in cis with the beta-globin gene promoter driving a lacZ reporter gene were studied. Expression of the lacZ transgene was assayed by measuring beta-galactosidase enzyme activity in fetal liver extracts, the levels of which correlated with the quantity of lacZ mRNA determined using RNase protection assays. Unexpectedly, expression of the lacZ transgene was found to show strong position effects, varying as much as 700-fold per transgene copy. These position effects occurred even if the whole beta-globin gene was incorporated as part of the lacZ reporter gene. Moreover, DNase I-hypersensitive sites appeared in the transgene LCR in high expressing but not in low expressing lines, suggesting that the LCR itself was position dependent. In contrast, MEL cell clones, in which transcriptionally active integration sites were selected for, gave < 13-fold variation in expression per copy of an LCR-lacZ construct. These results show that the lacZ reporter affects the ability of the LCR to activate chromatin in mice and that culture cells are not an adequate model for position-independent gene expression studies.
Indexed as
Identifiers
8670875PMC452030W141696687What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.