ArticleThe EMBO journal1996
Activation of the serum response factor by p65/NF-kappaB.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The trial behind it
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Who cites it
21 citing papers in PubMed, 65 citations in OpenAlex.
- SM22α deficiency: promoting vascular fibrosis via SRF-SMAD3-mediated activation ofResearch square · 2024Article
- CD148 Deficiency in Fibroblasts Promotes the Development of Pulmonary Fibrosis.American journal of respiratory and critical care medicine · 2021Article
- Sequence homology in eukaryotes (SHOE): interactive visual tool for promoter analysis.BMC genomics · 2018Article
- Biomaterial Scaffolds as Pre-metastatic Niche Mimics Systemically Alter the Primary Tumor and Tumor Microenvironment.Advanced healthcare materials · 2018Article
- Review
- Co-activation of nuclear factor-κB and myocardin/serum response factor conveys the hypertrophy signal of high insulin levels in cardiac myoblasts.The Journal of biological chemistry · 2014Article
- MKL1/2 and ELK4 co-regulate distinct serum response factor (SRF) transcription programs in macrophages.BMC genomics · 2014Article
- Inositol polyphosphate multikinase is a coactivator for serum response factor-dependent induction of immediate early genes.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Review
- Myocardin inhibits cellular proliferation by inhibiting NF-kappaB(p65)-dependent cell cycle progression.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Protein kinase casein kinase 2 mediates inhibitor-kappaB kinase and aberrant nuclear factor-kappaB activation by serum factor(s) in head and neck squamous carcinoma cells.Cancer research · 2006Article
- Coordinated binding of NF-kappaB family members in the response of human cells to lipopolysaccharide.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- NF-kappaB and AP-1 connection: mechanism of NF-kappaB-dependent regulation of AP-1 activity.Molecular and cellular biology · 2004Article
- Expression profiling of serum inducible genes identifies a subset of SRF target genes that are MKL dependent.BMC molecular biology · 2004Article
- NF-kappaB protects from the lysosomal pathway of cell death.The EMBO journal · 2003Article
- The mechanism of complex formation between Fli-1 and SRF transcription factors.Nucleic acids research · 2000Article
- Novel roles of specific isoforms of protein kinase C in activation of the c-fos serum response element.Molecular and cellular biology · 1999Article
- Serum response factor is essential for mesoderm formation during mouse embryogenesis.The EMBO journal · 1998Article
- The human cytomegalovirus UL55 (gB) and UL75 (gH) glycoprotein ligands initiate the rapid activation of Sp1 and NF-kappaB during infection.Journal of virology · 1997Article
- Constitutive activation of NF-kappaB during progression of breast cancer to hormone-independent growth.Molecular and cellular biology · 1997Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study demonstrates that the NF-kappaB subunit p65 can act like an accessory protein for the serum response factor (SRF) in transfection assays. p65 functionally synergizes with SRF to activate the transcription of a reporter construct dependent only on the serum response element (SRE). The synergy of the two factors requires neither a kappaB motif nor direct contact of p65 with DNA. Consistent with these results, a physical complex containing p65 and SRF is observed in vitro. Synergy of the factors is independent of the previously described activation domains present on p65, ruling out indirect effects of p65, but synergy is dependent on the activation domain of SRF. The complexing of p65 and SRF is mediated by a segment of the SRF DNA binding domain, a region of the protein which has also been reported to inhibit its own activation domain. We speculate that p65, upon direct or facilitated interaction with SRF, may relieve the inhibitory activity of this segment, thus enabling the activation domain of SRF to become fully functional. In contrast to p65, the p50 subunit of NF-kappaB does not interact significantly with SRF, either functionally or physically. The data suggest the intriguing possibility that NF-kappaB may participate in the regulation of SRE-dependent promoters, expanding the range of activities of this rapidly activatable transcription factor.
Indexed as
Identifiers
8670842PMC451904W93066617What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.