ArticleThe EMBO journal1996
Iron-regulated DNA binding by the AFT1 protein controls the iron regulon in yeast.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 127 papers.
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Who cites it
127 citing papers in PubMed, 338 citations in OpenAlex.
- Article
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- Next-generation stress-inducible Komagataella phaffii promoter variants.Microbial cell factories · 2025Article
- The Ferroxidase-Permease System for Transport of Iron Across Membranes: From Yeast to Humans.International journal of molecular sciences · 2025Review
- N88S seipin-related seipinopathy is a lipidopathy associated with loss of iron homeostasis.Cell communication and signaling : CCS · 2025Article
- The HMA-domain protein Ict1 is required for Ferroptosis in the rice blast fungus.Frontiers in plant science · 2025Article
- Siderophore Biosynthesis and Transport Systems in Model and Pathogenic Fungi.Journal of microbiology and biotechnology · 2024Review
- Iron Homeostatic Regulation in Saccharomyces cerevisiae: Introduction to a Computational Modeling Method.Methods in molecular biology (Clifton, N.J.) · 2024Review
- Regulatory and pathogenic mechanisms in response to iron deficiency and excess in fungi.Microbial biotechnology · 2023Review
- The Regulatory Hub of Siderophore Biosynthesis in the Phytopathogenic FungusJournal of fungi (Basel, Switzerland) · 2023Article
- Aft1 Nuclear Localization and Transcriptional Response to Iron Starvation Rely upon TORC2/Ypk1 Signaling and Sphingolipid Biosynthesis.International journal of molecular sciences · 2023Article
- Both human and soya bean ferritins highly improve the accumulation of bioavailable iron and contribute to extend the chronological life in budding yeast.Microbial biotechnology · 2022Article
- Effect of growth medium nitrogen and phosphorus on nutritional composition of Lemna minor (an alternative fish and poultry feed).BMC plant biology · 2022Article
- Biosynthesis Pathways, Transport Mechanisms and Biotechnological Applications of Fungal Siderophores.Journal of fungi (Basel, Switzerland) · 2021Review
- A pathogenic role for histone H3 copper reductase activity in a yeast model of Friedreich's ataxia.Science advances · 2021Article
- Sterol Composition Modulates the Response ofJournal of fungi (Basel, Switzerland) · 2021Article
- Promoter Architecture and Promoter Engineering inMetabolites · 2020Review
- Regulation of Iron Homeostasis and Use in Chloroplasts.International journal of molecular sciences · 2020Review
- Iron-sulfur cluster signaling: The common thread in fungal iron regulation.Current opinion in chemical biology · 2020Review
67 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Iron deprivation of Saccharomyces cerevisiae induces transcription of genes required for high-affinity iron uptake. AFT1 mediates this transcriptional control. In this report, the 5'-flanking region of FET3, which encodes a copper-dependent oxidase required for iron transport, was analyzed and found to contain a DNA sequence responsible for AFT1-regulated gene expression. AFT1 was capable of interacting specifically with this DNA sequence. A core element within this DNA sequence necessary for the binding of AFT1 was also determined. In vivo footprinting demonstrated occupancy of the AFT1 binding site in cells deprived of iron and not in cells grown in the presence of iron. Thus, the environmental signal resulting from iron deprivation was transduced through the regulated binding of AFT1 to the FET3 promoter, followed by the activation of transcription. A regulon of genes under the control of AFT1 could be defined. AFT1 was able to bind to a consensus binding site (PyPuCACCCPu) in the 5' region of FRE1, FRE2, FTR1, FTH1 and CCC2.
Indexed as
Identifiers
8670839PMC451901W1534809362What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.