ArticleThe EMBO journal1996
DNA-dependent protein kinase catalytic subunit: a target for an ICE-like protease in apoptosis.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 90 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
90 citing papers in PubMed, 346 citations in OpenAlex.
- Secrets of DNA-PKcs beyond DNA repair.NPJ precision oncology · 2024Review
- Neuroprotective Effect of Plasminogen Activator Inhibitor-1 Antagonist in the Rat Model of Mild Traumatic Brain Injury.Inflammation · 2021Article
- Relationship between thyroid tumor radiosensitivity and nuclear localization of DNA-dependent protein kinase catalytic subunit.Journal of radiation research · 2020Article
- Pigment epithelium-derived factor mediates retinal ganglion cell neuroprotection by suppression of caspase-2.Cell death & disease · 2019Article
- Vorinostat, a pan-HDAC inhibitor, abrogates productive HPV-18 DNA amplification.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Monoubiquitinated γ-H2AX: Abundant product and specific biomarker for non-apoptotic DNA double-strand breaks.Toxicology and applied pharmacology · 2018Article
- Proteasome inhibitor bortezomib is a novel therapeutic agent for focal radiation-induced osteoporosis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2018Article
- The DNA-dependent protein kinase: A multifunctional protein kinase with roles in DNA double strand break repair and mitosis.Progress in biophysics and molecular biology · 2015Review
- Preventing Damage Limitation: Targeting DNA-PKcs and DNA Double-Strand Break Repair Pathways for Ovarian Cancer Therapy.Frontiers in oncology · 2015Review
- Activated platelets rescue apoptotic cells via paracrine activation of EGFR and DNA-dependent protein kinase.Cell death & disease · 2014Article
- RNF144A, an E3 ubiquitin ligase for DNA-PKcs, promotes apoptosis during DNA damage.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Combined suppression of CASP2 and CASP6 protects retinal ganglion cells from apoptosis and promotes axon regeneration through CNTF-mediated JAK/STAT signalling.Brain : a journal of neurology · 2014Article
- The nuclear γ-H2AX apoptotic ring: implications for cancers and autoimmune diseases.Cellular and molecular life sciences : CMLS · 2014Review
- Unraveling the complexities of DNA-dependent protein kinase autophosphorylation.Molecular and cellular biology · 2014Article
- The N-terminal region of the DNA-dependent protein kinase catalytic subunit is required for its DNA double-stranded break-mediated activation.The Journal of biological chemistry · 2013Article
- Article
- Augmented generation of protein fragments during wakefulness as the molecular cause of sleep: a hypothesis.Protein science : a publication of the Protein Society · 2012Article
- Functional interplay between caspase cleavage and phosphorylation sculpts the apoptotic proteome.Cell · 2012Article
- Sublethal doses of β-amyloid peptide abrogate DNA-dependent protein kinase activity.The Journal of biological chemistry · 2012Article
- Pharmacological inhibition of caspase-2 protects axotomised retinal ganglion cells from apoptosis in adult rats.PloS one · 2012Article
30 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 1 institution in 1 country.
Funding
Abstract
Radiosensitive cell lines derived from X-ray cross complementing group 5 (XRCC5), SCID mice and a human glioma cell line lack components of the DNA-dependent protein kinase, DNA-PK, suggesting that DNA-PK plays an important role in DNA double-strand break repair. Another enzyme implicated in DNA repair, poly(ADP-ribose) polymerase, is cleaved and inactivated during apoptosis, suggesting that some DNA repair proteins may be selectively targeted for destruction during apoptosis. Here we demonstrate that DNA-PKcs, the catalytic subunit of DNA-PK, is preferentially degraded after the exposure of different cell types to a variety of agents known to cause apoptosis. However, Ku, the DNA-binding component of the enzyme, remains intact. Degradation of DNA-PKcs was accompanied by loss of DNA-PK activity. One cell line resistant to etoposide-induced apoptosis failed to show degradation of DNA-PKcs. Protease inhibitor data implicated an ICE-like protease in the cleavage of DNA-PKcs, and it was subsequently shown that the cysteine protease CPP32, but not Mch2alpha, ICE or TX, cleaved purified DNA-PKcs into three fragments of comparable size with those observed in cells undergoing apoptosis. Cleavage sites in DNA-PKcs, determined by antibody mapping and microsequencing, were shown to be the same for CPP32 cleavage and for cleavage catalyzed by extracts from cells undergoing apoptosis. These observations suggest that DNA-PKcs is a critical target for proteolysis by an ICE-like protease during apoptosis.
Indexed as
Identifiers
8670824PMC451880W25687112What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.