ArticleThe EMBO journal1996
Myristoylated and non-myristoylated forms of the pH sensor protein hisactophilin II: intracellular shuttling to plasma membrane and nucleus monitored in real time by a fusion with green fluorescent protein.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 72 citations in OpenAlex.
- Surface electrostatic networks control hydrophobic core remodeling in a pH-dependent switching protein.bioRxiv : the preprint server for biology · 2026Article
- A fine balance of hydrophobic-electrostatic communication pathways in a pH-switching protein.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Phospholipids containing ether-bound hydrocarbon-chains are essential for efficient phagocytosis and neutral lipids of the ester-type perturb development inBiology open · 2020Article
- A recombinant antibody toolbox for Dictyostelium discoideum.BMC research notes · 2020Article
- Cell spread area and traction forces determine myosin-II-based cortex thickness regulation.Biochimica et biophysica acta. Molecular cell research · 2019Article
- The Cytoskeleton-A Complex Interacting Meshwork.Cells · 2019Review
- The actin cortex at a glance.Journal of cell science · 2018Review
- In pursuit of the mechanics that shape cell surfaces.Nature physics · 2018Article
- Actin cortex architecture regulates cell surface tension.Nature cell biology · 2017Article
- Dissecting the actin cortex density and membrane-cortex distance in living cells by super-resolution microscopy.Journal of physics D: Applied physics · 2017Article
- The Dictyostelium discoideum GPHR ortholog is an endoplasmic reticulum and Golgi protein with roles during development.Eukaryotic cell · 2015Article
- A histidine-rich linker region in peptidylglycine α-amidating monooxygenase has the properties of a pH sensor.The Journal of biological chemistry · 2014Article
- Dictyostelium discoideum Dgat2 can substitute for the essential function of Dgat1 in triglyceride production but not in ether lipid synthesis.Eukaryotic cell · 2014Article
- Dictyostelium lipid droplets host novel proteins.Eukaryotic cell · 2013Article
- Monitoring actin cortex thickness in live cells.Biophysical journal · 2013Article
- Applicability of green fluorescence protein in the study of endothelin converting enzyme-1c trafficking.Protein science : a publication of the Protein Society · 2013Article
- Considering protonation as a posttranslational modification regulating protein structure and function.Annual review of biophysics · 2013Review
- The testis-specific Cα2 subunit of PKA is kinetically indistinguishable from the common Cα1 subunit of PKA.BMC biochemistry · 2011Article
- Protein kinase A type I activates a CRE-element more efficiently than protein kinase A type II regardless of C subunit isoform.BMC biochemistry · 2011Article
- Energetics and mechanisms of folding and flipping the myristoyl switch in the {beta}-trefoil protein, hisactophilin.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hisactophilins are myristoylated proteins that are rich in histidine residues and known to exist in Dictyostelium cells in a plasma membrane-bound and a soluble cytoplasmic state. Intracellular translocation of these proteins in response to pH changes was monitored using hisactophilin fusions with green fluorescent protein (GFP) and confocal laser scanning microscopy. Both the normal and a mutated non-myristoylated fusion protein shuffled within the cells in a pH-dependent manner. After lowering the pH, these proteins translocated within minutes between the cytoplasm, the plasma membrane and the nucleus. The role of histidine clusters on the surface of hisactophilin molecules in binding of the proteins to the plasma membrane and in their transfer to the nucleus is discussed on the basis of a pH switch mechanism.
Indexed as
Identifiers
8670794PMC450234W162149502What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.