ArticleThe Biochemical journal1996
A limited upstream region of the human sucrase-isomaltase gene confers glucose-regulated expression on a heterologous gene.
Article in The Biochemical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Fine-tuning and autoregulation of the intestinal determinant and tumor suppressor homeobox gene CDX2 by alternative splicing.Cell death and differentiation · 2017Article
- Identification of an intestine-specific promoter and inducible expression of bacterial α-galactosidase in mammalian cells by a lac operon system.Journal of animal science and biotechnology · 2012Article
- Phosphorylation of the homeotic tumor suppressor Cdx2 mediates its ubiquitin-dependent proteasome degradation.Oncogene · 2005Article
- Glucose and thyroid hormone co-regulate the expression of the intestinal fructose transporter GLUT5.The Biochemical journal · 1999Article
- Two HNF-1 binding sites govern the glucose repression of the human sucrase-isomaltase promoter.The Biochemical journal · 1998Article
- Dietary carbohydrates enhance lactase/phlorizin hydrolase gene expression at a transcription level in rat jejunum.The Biochemical journal · 1998Article
Corrections and comments
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Authors and funding
7 authors.
Funding
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Abstract
We have previously shown that glucose can exert a repressive effect on the transcription of the sucrase-isomaltase (SI) gene in the differentiated enterocyte-like human colon carcinoma cell lines HT-29 and Caco-2. To characterize the region through which glucose exerts this effect, three different-length fragments of the 5'-flanking region of the human SI gene were linked to the reporter gene luciferase in an episomal vector carrying a hygromycin resistance gene. These fragments were used for transfection into a clone of the Caco-2 cell line, PF11, which has high glucose consumption and only expresses SI at high levels when cultured in the presence of a low supply of glucose. By using the stably transformed PF11 cells grown either in standard high glucose (25 mM) or in low glucose (1 mM) it was possible to show that the smallest fragment of the SI promoter, extending from bases -370 to +30, contains all the information required for the glucose repression of the reporter gene luciferase.
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