ArticleThe Biochemical journal1996
Sodium-proton exchange stimulates Ca2+ release from acidocalcisomes of Trypanosoma brucei.
Article in The Biochemical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 67 citations in OpenAlex.
- Monocellular and Multicellular Parasites Infesting Humans: A Review of Calcium Ion Mechanisms.Biomedicines · 2025Review
- Advances in the cellular biology, biochemistry, and molecular biology of acidocalcisomes.Microbiology and molecular biology reviews : MMBR · 2024Review
- New insights into the role of acidocalcisomes in trypanosomatids.The Journal of eukaryotic microbiology · 2022Review
- The IPBiochimica et biophysica acta. Molecular cell research · 2021Review
- Emerging therapeutic targets for treatment of leishmaniasis.Expert opinion on therapeutic targets · 2018Review
- A Calcium/Cation Exchanger Participates in the Programmed Cell Death andFrontiers in cellular and infection microbiology · 2018Article
- pH regulation in glycosomes of procyclic formThe Journal of biological chemistry · 2017Article
- Acidocalcisomes of eukaryotes.Current opinion in cell biology · 2016Review
- A Novel Polyclonal Antiserum against Toxoplasma gondii Sodium Hydrogen Exchanger 1.The Korean journal of parasitology · 2016Article
- Polyphosphate and acidocalcisomes.Biochemical Society transactions · 2016Review
- Proteomic analysis of the acidocalcisome, an organelle conserved from bacteria to human cells.PLoS pathogens · 2014Article
- Acidocalcisome is required for autophagy in Trypanosoma brucei.Autophagy · 2014Article
- New insights into roles of acidocalcisomes and contractile vacuole complex in osmoregulation in protists.International review of cell and molecular biology · 2013Review
- Acidocalcisomes.Cell calcium · 2011Review
- Calcium- and polyphosphate-containing acidic granules of sea urchin eggs are similar to acidocalcisomes, but are not the targets for NAADP.The Biochemical journal · 2010Article
- Evolution of acidocalcisomes and their role in polyphosphate storage and osmoregulation in eukaryotic microbes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2010Review
- Ionophore-resistant mutant of Toxoplasma gondii reveals involvement of a sodium/hydrogen exchanger in calcium regulation.The Journal of cell biology · 2004Article
- Dynamics of polymorphism of acidocalcisomes in Leishmania parasites.Histochemistry and cell biology · 2004Article
- A plasma membrane-type Ca(2+)-ATPase co-localizes with a vacuolar H(+)-pyrophosphatase to acidocalcisomes of Toxoplasma gondii.The EMBO journal · 2001Article
- Characterization of a vacuolar pyrophosphatase in Trypanosoma brucei and its localization to acidocalcisomes.Molecular and cellular biology · 1999Article
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Authors and funding
2 authors at 1 institution in 1 country.
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Abstract
Acidocalcisomes are acidic vacuoles present in trypanosomatids that contain a considerable fraction of intracellular Ca2+ [Vercesi, Moreno and Docampo (1994) Biochem. J. 304, 227-233; Scott, Moreno and Docampo (1995) Biochem. J. 310, 789-794; Docampo, Scott, Vercesi and Moreno (1995) Biochem. J. 310, 1005-1012]. The data presented here indicate that Na+ stimulates Ca2+ release from the acidocalcisomes of digitonin-permeabilized Trypanosoma brucei procyclic trypomastigotes in a dose-dependent fashion, this effect being enhanced by increasing pH of the medium from 7.0 to 7.8. The hypothesis that this Na+ effect was mediated by alkalinization of the acidocalcisomes via a Na+/H+ antiporter was supported by experiments showing that Na+ promotes release of Acridine Orange previously accumulated in these vacuoles. This putative antiporter did not transport Li+ and was not sensitive to the amiloride analogue 5-(N-ethyl-N-isopropyl)amiloride. Addition of the Na+/H+ ionophore monensin to intact cells loaded with fura 2, in the nominal absence of extracellular Ca2+ to preclude Ca2+ entry, was followed by an increase in cytosolic Ca2+ concentration ([Ca2+]i), which was more accentuated in the presence of extracellular Na+. An increase in intracellular pH (pHi) of BCECF-loaded cells was detected after addition of monensin in the presence of extracellular Na+, whereas a dramatic decrease in pHi was detected in its absence, thus indicating the presence of a significant amount of releasable protons in the acidic compartments. These results are consistent with the presence of a Na+/H+ antiporter in the acidocalcisomes that could be involved in the regulation of pH1 and [Ca2+]1 in these parasites.
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