ArticleThe Biochemical journal1996
Optimized heterologous expression of the human zinc enzyme glyoxalase I.
Article in The Biochemical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 58 citations in OpenAlex.
- Evolutionary Aspects of the Oxido-Reductive Network of Methylglyoxal.Journal of molecular evolution · 2021Article
- Targeting Metalloenzymes for Therapeutic Intervention.Chemical reviews · 2019Review
- Article
- Defense against Reactive Carbonyl Species Involves at Least Three Subcellular Compartments Where Individual Components of the System Respond to Cellular Sugar Status.The Plant cell · 2017Article
- Genome-wide analysis and expression profiling of glyoxalase gene families in soybean (Glycine max) indicate their development and abiotic stress specific response.BMC plant biology · 2016Article
- Article
- Methylglyoxal in cells elicits a negative feedback loop entailing transglutaminase 2 and glyoxalase 1.Redox biology · 2014Article
- Structural variation in bacterial glyoxalase I enzymes: investigation of the metalloenzyme glyoxalase I from Clostridium acetobutylicum.The Journal of biological chemistry · 2011Article
- Posttranslational modification of human glyoxalase 1 indicates redox-dependent regulation.PloS one · 2010Article
- Comparative genomic analyses of nickel, cobalt and vitamin B12 utilization.BMC genomics · 2009Article
- Enhancing salt tolerance in a crop plant by overexpression of glyoxalase II.Transgenic research · 2008Article
- Distinct classes of glyoxalase I: metal specificity of the Yersinia pestis, Pseudomonas aeruginosa and Neisseria meningitidis enzymes.The Biochemical journal · 2004Article
- A trypanothione-dependent glyoxalase I with a prokaryotic ancestry in Leishmania major.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Investigation of metal binding and activation of Escherichia coli glyoxalase I: kinetic, thermodynamic and mutagenesis studies.The Biochemical journal · 2004Article
- A stress-responsive glyoxalase I from the parasitic nematode Onchocerca volvulus.The Biochemical journal · 2001Article
- Identification of candidate coding region single nucleotide polymorphisms in 165 human genes using assembled expressed sequence tags.Genome research · 1999Article
- Mutagenesis of residue 157 in the active site of human glyoxalase I.The Biochemical journal · 1997Article
- Crystal structure of human glyoxalase I--evidence for gene duplication and 3D domain swapping.The EMBO journal · 1997Article
- Molecular cloning and characterization of the thiolesterase glyoxalase II from Arabidopsis thaliana.The Biochemical journal · 1997Article
- Distribution of glyoxalase I polymorphism among Zuni Indians: the Zuni Kidney Project.Journal of diabetes and its complicationsArticle
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA coding for human glyoxalase I was isolated from a HeLa cell cDNA library by means of PCR. The deduced amino acid sequence differs form previously isolated sequences in that a glutamic acid replaces an alanine in position 111. This variant cDNA may represent the more acidic isoform of glyoxalase I originally identified at the protein level. An expression clone was constructed for high-level production of glyoxalase I in Escherichia coli. For optimal yield of the recombinant protein, silent random mutations were introduced in the cDNA coding region. Antisera against human glyoxalase I were used to select a high-level expression clone. This clone afforded 60 mg of purified enzyme per litre of culture medium. Addition of a zinc salt to the culture medium was essential to obtain an active enzyme and a stoicheiometric metal content. The functional characterization of the recombinant enzyme included determination of kinetic constants for methylglyoxal, phenylglyoxal and p-phenylphenylglyoxal, as well as inhibition studies. The kinetic properties of recombinant glyoxalase I were indistinguishable from those of the enzyme purified from human tissues.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.