ArticleNucleic acids research1996
Positively charged oligonucleotides overcome potassium-mediated inhibition of triplex DNA formation.
Article in Nucleic acids research, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Three's a crowd - stabilisation, structure, and applications of DNA triplexes.Chemical science · 2022Review
- Cationic oligonucleotide derivatives and conjugates: A favorable approach for enhanced DNA and RNA targeting oligonucleotides.Beilstein journal of organic chemistry · 2021Review
- The free solution mobility of DNA and other analytes varies as the logarithm of the fractional negative charge.Electrophoresis · 2014Article
- Improved bioactivity of G-rich triplex-forming oligonucleotides containing modified guanine bases.Artificial DNA, PNA & XNA · 2014Article
- Invader LNA: efficient targeting of short double stranded DNA.Organic & biomolecular chemistry · 2010Article
- Purine twisted-intercalating nucleic acids: a new class of anti-gene molecules resistant to potassium-induced aggregation.Nucleic acids research · 2008Article
- Bioconjugation of oligonucleotides for treating liver fibrosis.Oligonucleotides · 2007Review
- Reduction of XNkx2-10 expression leads to anterior defects and malformation of the embryonic heart.Mechanisms of development · 2006Article
- Monitoring denaturation behaviour and comparative stability of DNA triple helices using oligonucleotide-gold nanoparticle conjugates.Nucleic acids research · 2004Article
- Therapeutic modulation of endogenous gene function by agents with designed DNA-sequence specificities.Nucleic acids research · 2003Review
- Cationic phosphoramidate alpha-oligonucleotides efficiently target single-stranded DNA and RNA and inhibit hepatitis C virus IRES-mediated translation.Nucleic acids research · 2003Article
- The potential for gene repair via triple helix formation.The Journal of clinical investigation · 2003Review
- Targeted elimination of zygotic messages in Xenopus laevis embryos by modified oligonucleotides possessing terminal cationic linkages.Nucleic acids research · 2000Article
- Understanding oligonucleotide-mediated inhibition of gene expression in Xenopus laevis oocytes.Nucleic acids research · 2000Article
- Thermodynamic and kinetic studies of the formation of triple helices between purine-rich deoxyribo-oligonucleotides and the promoter region of the human c-src proto-oncogene.Nucleic acids research · 1998Article
- Poly(L-lysine)-graft-dextran copolymer: amazing effects on triplex stabilization under physiological pH and ionic conditions (in vitro).Nucleic acids research · 1998Article
- Zwitterionic oligodeoxyribonucleotide N3'-->P5' phosphoramidates: synthesis and properties.Nucleic acids research · 1998Article
- Antiparallel polypurine phosphorothioate oligonucleotides form stable triplexes with the rat alpha1(I) collagen gene promoter and inhibit transcription in cultured rat fibroblasts.Nucleic acids research · 1997Article
- Divalent transition metal cations counteract potassium-induced quadruplex assembly of oligo(dG) sequences.Nucleic acids research · 1997Article
- Selective inhibition of the human tie-1 promoter with triplex-forming oligonucleotides targeted to Ets binding sites.Molecular medicine (Cambridge, Mass.)Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The formation of triplex DNA using unmodified, purine-rich oligonucleotides (ODNs) is inhibited by physiologic levels of potassium. Changing negative phosphodiester bonds in a triplex forming oligonucleotide (TFO) to neutral linkages causes a small increase in triplex formation. When phosphodiester bonds in a TFO are converted to positively-charged linkages the formation of triplex DNA increases dramatically. In the absence of KCl, a 17mer TFO containing 11 positively-charged linkages at a concentration of 0.2 microM converts essentially all of a 30 bp target duplex to a triplex. Less than 15% of the target duplex is shifted by 2 microMolar of the unmodified TFO. In 130 mM KCl, triplex formation is undetectable using the unmodified TFO, while triplex formation is nearly complete with 2 microM positively-charged TFO. With increasing potassium, TFOs containing a higher proportion of modified linkages show enhanced triplex formation compared with those less modified. In contrast with unmodified TFOs, triplex formation with more heavily modified TFOs can occur in the absence of divalent cations. We conclude that replacement of phosphodiester bonds with positively-charged phosphoramidate linkages results in more efficient triplex formation, suggesting that these compounds may prove useful for in vivo applications.
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