ArticleNucleic acids research1996
Extrachromosomal recombination occurs efficiently in cells defective in various DNA repair systems.
Article in Nucleic acids research, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 26 citations in OpenAlex.
- A comparison of synthetic oligodeoxynucleotides, DNA fragments and AAV-1 for targeted episomal and chromosomal gene repair.BMC biotechnology · 2009Article
- Inhibition of poly(ADP-ribose)polymerase does not affect the recombination events in CHO xrs6 and wild type cells.Radiation and environmental biophysics · 2006Article
- Extrachromosomal genes: a powerful tool in gene targeting approaches.Gene therapy · 2002Article
- Restriction enzymes increase efficiencies of illegitimate DNA integration but decrease homologous integration in mammalian cells.Nucleic acids research · 2001Article
- Early embryonic lethality in PARP-1 Atm double-mutant mice suggests a functional synergy in cell proliferation during development.Molecular and cellular biology · 2001Article
- The controlling role of ATM in homologous recombinational repair of DNA damage.The EMBO journal · 2000Article
- Poly(ADP-ribosyl)ation reactions in the regulation of nuclear functions.The Biochemical journal · 1999Review
- Arabidopsis thaliana mutants altered in homologous recombination.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- PARP is important for genomic stability but dispensable in apoptosis.Genes & development · 1997Article
- Review
- The role of extracellular DNA in the stability and variability of cell genomes.Doklady. Biochemistry and biophysicsArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A series of different frameshift mutations of a firefly luciferase reporter plasmid was created so that no activity was obtained when they were transfected into mammalian cells. Co-transfection of these constructs with short fragments of the original sequence resulted in luciferase activity in different cell lines (A-549, NIH 3T3 and Jurkat). The level of this activity was dependent on the length of the fragment, regardless of cell line examined. Two different transfection techniques (lipofection and adenovirus-enhanced gene transfer) gave similar results. It was shown by polymerase chain reaction that expression of detectable luciferase required recombination of the transfected molecules. Cells with defined defects in DNA repair pathways were examined for their ability to perform this extrachromosomal recombination. Cells lacking normal Ku p80, (ADP-ribosyl)transferase, MLH1 or XP-C were all capable of restoring expression to the frameshifted constructs. Given the pivotal roles of the above molecules in the pathways of DNA repair, it seems that this recombination derives from a different activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.