ArticleMolecular and cellular biology1996
Analysis of functional domain organization in DNA topoisomerase II from humans and Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 48 citations in OpenAlex.
- Yeast Tools for Studying Type II Topoisomerases in Budding Yeast.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Aclarubicin Reduces the Nuclear Mobility of Human DNA Topoisomerase IIβ.International journal of molecular sciences · 2024Article
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- The Impact of the C-Terminal Region on the Interaction of Topoisomerase II Alpha with Mitotic Chromatin.International journal of molecular sciences · 2019Article
- The Roles of DNA Topoisomerase IIβ in Transcription.International journal of molecular sciences · 2018Review
- Granzyme M targets topoisomerase II alpha to trigger cell cycle arrest and caspase-dependent apoptosis.Cell death and differentiation · 2014Article
- A novel chromatin tether domain controls topoisomerase IIα dynamics and mitotic chromosome formation.The Journal of cell biology · 2013Article
- Chiral discrimination and writhe-dependent relaxation mechanism of human topoisomerase IIα.The Journal of biological chemistry · 2013Article
- Centromere-associated topoisomerase activity in bloodstream form Trypanosoma brucei.Nucleic acids research · 2011Article
- The use of divalent metal ions by type II topoisomerases.Metallomics : integrated biometal science · 2010Review
- Bimodal recognition of DNA geometry by human topoisomerase II alpha: preferential relaxation of positively supercoiled DNA requires elements in the C-terminal domain.Biochemistry · 2008Article
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- HMGB1 interacts with human topoisomerase IIalpha and stimulates its catalytic activity.Nucleic acids research · 2007Article
- Nuclear interactions of topoisomerase II alpha and beta with phospholipid scramblase 1.Nucleic acids research · 2007Article
- C-terminal regions of topoisomerase IIalpha and IIbeta determine isoform-specific functioning of the enzymes in vivo.Nucleic acids research · 2007Article
- Impact of the C-terminal domain of topoisomerase IIalpha on the DNA cleavage activity of the human enzyme.Biochemistry · 2005Article
- Modulation of drug sensitivity in yeast cells by the ATP-binding domain of human DNA topoisomerase IIalpha.Nucleic acids research · 2003Article
- Functional dissection of the C-terminal domain of type II DNA topoisomerase from the kinetoplastid hemoflagellate Leishmania donovani.Nucleic acids research · 2003Article
- Cloning, functional analysis and post-transcriptional regulation of a type II DNA topoisomerase from Leishmania infantum. A new potential target for anti-parasite drugs.Nucleic acids research · 2003Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The functional domain structure of human DNA topoisomerase IIalpha and Saccharomyces cerevisiae DNA topoisomerase II was studied by investigating the abilities of insertion and deletion mutant enzymes to support mitotic growth and catalyze transitions in DNA topology in vitro. Alignment of the human topoisomerase IIalpha and S. cerevisiae topoisomerase II sequences defined 13 conserved regions separated by less conserved or differently spaced sequences. The spatial tolerance of the spacer regions was addressed by insertion of linkers. The importance of the conserved regions was assessed through deletion of individual domains. We found that the exact spacing between most of the conserved domains is noncritical, as insertions in the spacer regions were tolerated with no influence on complementation ability. All conserved domains, however, are essential for sustained mitotic growth of S. cerevisiae and for enzymatic activity in vitro. A series of topoisomerase II carboxy-terminal truncations were investigated with respect to the ability to support viability, cellular localization, and enzymatic properties. The analysis showed that the divergent carboxy-terminal region of human topoisomerase IIalpha is dispensable for catalytic activity but contains elements that specifically locate the protein to the nucleus.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.