ArticleMolecular and cellular biology1996
Non-Mendelian, heritable blocks to DNA rearrangement are induced by loading the somatic nucleus of Tetrahymena thermophila with germ line-limited DNA.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 116 citations in OpenAlex.
- The Conjusome-A Transient Organelle Linking Genome Rearrangements in the Parental and Developing Macronuclei.Microorganisms · 2023Article
- Temperature affects the repeatability of evolution in the microbial eukaryoteEcology and evolution · 2021Article
- Roles of Noncoding RNAs in Ciliate Genome Architecture.Journal of molecular biology · 2020Review
- Boundaries of eliminated heterochromatin of Tetrahymena are positioned by the DNA-binding protein Ltl1.Nucleic acids research · 2019Article
- Functional Proteomics of Nuclear Proteins inGenes · 2019Review
- DRH1, a p68-related RNA helicase gene, is required for chromosome breakage in Tetrahymena.Biology open · 2016Article
- A Parallel G Quadruplex-Binding Protein Regulates the Boundaries of DNA Elimination Events of Tetrahymena thermophila.PLoS genetics · 2016Article
- Chromodomain protein Tcd1 is required for macronuclear genome rearrangement and repair in Tetrahymena.Scientific reports · 2015Article
- Programmed Genome Rearrangements in the Ciliate Oxytricha.Microbiology spectrum · 2014Review
- Genome-wide analysis of genetic and epigenetic control of programmed DNA deletion.Nucleic acids research · 2014Article
- An expanding world of small RNAs.Developmental cell · 2014Article
- Epigenetics of ciliates.Cold Spring Harbor perspectives in biology · 2013Review
- Dogma derailed: the many influences of RNA on the genome.Molecular cell · 2013Review
- Selecting one of several mating types through gene segment joining and deletion in Tetrahymena thermophila.PLoS biology · 2013Article
- Article
- Biased transcription and selective degradation of small RNAs shape the pattern of DNA elimination in Tetrahymena.Genes & development · 2012Article
- Developmentally programmed, RNA-directed genome rearrangement in Tetrahymena.Development, growth & differentiation · 2012Review
- Genome-Scale Analysis of Programmed DNA Elimination Sites in Tetrahymena thermophila.G3 (Bethesda, Md.) · 2011Article
- Keeping the soma free of transposons: programmed DNA elimination in ciliates.The Journal of biological chemistry · 2011Review
- Programmed DNA elimination in Tetrahymena: a small RNA-mediated genome surveillance mechanism.Advances in experimental medicine and biology · 2011Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 2 countries.
Funding
Abstract
Site-specific DNA deletion occurs at thousands of sites within the genome during macronuclear development of Tetrahymena thermophila. These deletion elements are usually not detected in macronuclear chromosomes. We have interfered with the normal deletion of two of these elements, the adjacent M and R elements, by loading vegetative macronuclei with these elements prior to sexual conjugation. Transformed cell lines containing the exogenous M or R element, carried on high-copy-number vectors containing genes encoding rRNA within parental (old) macronuclei, consistently failed to excise chromosomal copies of the M or R element during formation of new macronuclei. Little or no interference with the deletions of adjacent elements or of unlinked elements was observed. The micronucleus (germ line)-limited region of each element was sufficient to inhibit specific DNA deletion. This interference with DNA deletion usually is manifested as a cytoplasmic dominant trait: deletion elements present in the old macronucleus of one partner of a mating pair were sufficient to inhibit deletion occurring in the other partner. Remarkably, the failure to excise these elements became a non-Mendelian, inheritable trait in the next generation and did not require the high copy number of exogenously introduced elements. The introduction of exogenous deletion elements into parental macronuclei provides us with an epigenetic means to establish a heritable pattern of DNA rearrangement.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.