ArticleMolecular and cellular biology1996
Identification of overlapping DNA-binding and centromere-targeting domains in the human kinetochore protein CENP-C.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
65 citing papers in PubMed, 123 citations in OpenAlex.
- Centromeres drive and take a break.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Review
- Article
- Plant kinetochore complex: composition, function, and regulation.Frontiers in plant science · 2024Review
- Population analyses of mosaic X chromosome loss identify genetic drivers and widespread signatures of cellular selection.medRxiv : the preprint server for health sciences · 2023Article
- Kinetochore Architecture Employs Diverse Linker Strategies Across Evolution.Frontiers in cell and developmental biology · 2022Review
- Diverse mechanisms of centromere specification.Current biology : CB · 2021Review
- A genetic memory initiates the epigenetic loop necessary to preserve centromere position.The EMBO journal · 2020Article
- Centromere and Pericentromere Transcription: Roles and Regulation … in Sickness and in Health.Frontiers in genetics · 2018Review
- Molecular basis of CENP-C association with the CENP-A nucleosome at yeast centromeres.Genes & development · 2017Article
- Centromeres are maintained by fastening CENP-A to DNA and directing an arginine anchor-dependent nucleosome transition.Nature communications · 2017Article
- Drosophila Nnf1 paralogs are partially redundant for somatic and germ line kinetochore function.Chromosoma · 2017Article
- A Molecular View of Kinetochore Assembly and Function.Biology · 2017Review
- Targeting of Arabidopsis KNL2 to Centromeres Depends on the Conserved CENPC-k Motif in Its C Terminus.The Plant cell · 2017Article
- Chromosome biorientation produces hundreds of piconewtons at a metazoan kinetochore.Nature communications · 2016Article
- The unconventional kinetoplastid kinetochore: from discovery toward functional understanding.Biochemical Society transactions · 2016Review
- Differential Binding Partners of the Mis18α/β YIPPEE Domains Regulate Mis18 Complex Recruitment to Centromeres.Cell reports · 2016Article
- Article
- CENP-C is a blueprint for constitutive centromere-associated network assembly within human kinetochores.The Journal of cell biology · 2015Article
- Discovering centromere proteins: from cold white hands to the A, B, C of CENPs.Nature reviews. Molecular cell biology · 2015Article
- Stable complex formation of CENP-B with the CENP-A nucleosome.Nucleic acids research · 2015Article
5 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The kinetochore in eukaryotes serves as the chromosomal site of attachment for microtubules of the mitotic spindle and directs the movements necessary for proper chromosome segregation. In mammalian cells, the kinetochore is a highly differentiated trilaminar structure situated at the surface of the centromeric heterochromatin. CENP-C is a basic, DNA-binding protein that localizes to the inner kinetochore plate, the region that abuts the heterochromatin. Microinjection experiments using antibodies specific for CENP-C have demonstrated that this protein is required for the assembly and/or stability of the kinetochore as well as for a timely transition through mitosis. From these observations, it has been suggested that CENP-C is a structural protein that is involved in the organization or the kinetochore. In this report, we wished to identify and map the functional domains of CENP-C. Analysis of CENP-C truncation mutants expressed in vivo demonstrated that CENP-C possesses an autonomous centromere-targeting domain situated at the central region of the CENP-C polypeptide. Similarly, in vitro assays revealed that a region of CENP-C with the ability to bind DNA is also located at the center of the CENP-C molecule, where it overlaps the centromere-targeting domain.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.