ArticleMolecular and cellular biology1996
Intron retention generates a novel isoform of the murine vitamin D receptor that acts in a dominant negative way on the vitamin D signaling pathway.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 83 citations in OpenAlex.
- Supportive treatment of vascular dysfunction in pediatric subjects with obesity: the OBELIX study.Nutrition & diabetes · 2022Trial
- Vitamin D Receptor in Cancer: Biological Functions, Mechanistic Insights, and Clinical Relevance.Cancer management and research · 2026Review
- Effect of Vitamin D Supplementation on Inflammatory Markers in Obese Patients with Acute and Chronic Orthopedic Conditions.Nutrients · 2024Article
- Vitamin D and Endothelial Function.Nutrients · 2020Review
- Hypoxia is a Key Driver of Alternative Splicing in Human Breast Cancer Cells.Scientific reports · 2017Article
- Article
- Human glucocorticoid receptor isoform beta: recent understanding of its potential implications in physiology and pathophysiology.Cellular and molecular life sciences : CMLS · 2009Review
- Naturally occurring C-terminal splice variants of nuclear receptors.Nuclear receptor signaling · 2009Review
- Alternative splicing and caspase-mediated cleavage generate antagonistic variants of the stress oncoprotein LEDGF/p75.Molecular cancer research : MCR · 2008Article
- Transrepression by a liganded nuclear receptor via a bHLH activator through co-regulator switching.The EMBO journal · 2004Article
- Alternatively spliced isoforms of the human constitutive androstane receptor.Nucleic acids research · 2003Article
- A dominant-negative isoform lacking exons 11 and 12 of the human hypoxia-inducible factor-1alpha gene.The Biochemical journal · 2002Article
- Cloning and characterization of two novel thyroid hormone receptor beta isoforms.Molecular and cellular biology · 2000Article
- Differential and inefficient splicing of a broadly expressed Drosophila erect wing transcript results in tissue-specific enrichment of the vital EWG protein isoform.Molecular and cellular biology · 1999Article
- Mutations in a dominant-negative isoform correlate with phenotype in inherited cardiac arrhythmias.American journal of human genetics · 1999Article
- Selective interaction of vitamin D receptor with transcriptional coactivators by a vitamin D analog.Molecular and cellular biology · 1999Article
- Multiple intron retention occurs in tumor cell CD44 mRNA processing.The American journal of pathology · 1998Article
- Multiple promoters direct the tissue-specific expression of novel N-terminal variant human vitamin D receptor gene transcripts.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Molecular cloning and characterization of human estrogen receptor betacx: a potential inhibitor ofestrogen action in human.Nucleic acids research · 1998Article
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Authors and funding
12 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We identified and characterized a novel rat vitamin D receptor isoform (rVDR1), which retains intron 8 of the canonical VDR (rVDR0) during alternative splicing. In this isoform protein directed by the stop codon in this newly identified exon, a part of the ligand binding domain (86 amino acids) is truncated at the C-terminal end but contains 19 extra amino acids. The rVDR1 transcript was expressed at a level 1/15 to 1/20 of that of rVDR0 in the kidney and intestine in adult rats but not in embryos. The recombinant rVDR1 protein showed no ligand binding activity. Homo- and heterodimers of the recombinant rVDR0 and rVDR1 proteins bound to a consensus vitamin D response element (VDRE) but not to consensus response elements for thyroid hormone and retinoic acid. However, unlike rVDR0, rVDR1 did not form a heterodimeric complex with RXR on the VDRE. A transient expression assay showed that this isoform acted as a dominant negative receptor against rVDR0 transactivation. Interestingly, the dominant negative activities of rVDR1 differed among VDREs. Thus, the present study indicates that this new VDR isoform negatively modulates the vitamin D signaling pathway, through a particular set of target genes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.