ArticleMolecular and cellular biology1996
Site-specific methylation of the rat prolactin and growth hormone promoters correlates with gene expression.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 46 citations in OpenAlex.
- Exploring the influence of DNA methylation and single nucleotide polymorphisms of theFrontiers in genetics · 2023Article
- Unveiling the gene regulatory landscape in diseases through the identification of DNase I-hypersensitive sites.Biomedical reports · 2019Article
- Manipulation of the Growth Hormone-Insulin-Like Growth Factor (GH-IGF) Axis: A Treatment Strategy to Reverse the Effects of Early Life Developmental Programming.International journal of molecular sciences · 2017Review
- Cell Type-Specific Sexual Dimorphism in Rat Pituitary Gene Expression During Maturation.Biology of reproduction · 2015Article
- Epigenetic characterization of the growth hormone gene identifies SmcHD1 as a regulator of autosomal gene clusters.PloS one · 2014Article
- Determination of Methylated CpG Sites in the Promoter Region of Catechol-O-Methyltransferase (COMT) and their Involvement in the Etiology of Tobacco Smoking.Frontiers in psychiatry · 2010Article
- Distinct DNA methylation patterns characterize differentiated human embryonic stem cells and developing human fetal liver.Genome research · 2009Article
- Hypermethylation of hepatic Gck promoter in ageing rats contributes to diabetogenic potential.Diabetologia · 2008Article
- CpG methylation at the USF-binding site is important for the liver-specific transcription of the chipmunk HP-27 gene.The Biochemical journal · 2006Article
- Applying whole-genome studies of epigenetic regulation to study human disease.Cytogenetic and genome research · 2006Review
- Bisulfite genomic sequencing: systematic investigation of critical experimental parameters.Nucleic acids research · 2001Article
- Hypothalamic and hypophyseal regulation of growth hormone secretion.Cellular and molecular neurobiology · 1998Review
- Involvement of AP-2 in regulation of the R-FABP gene in the developing chick retina.Molecular and cellular biology · 1997Article
- DNA methylation inhibits elongation but not initiation of transcription in Neurospora crassa.Genes & development · 1997Article
- Chicken vigilin gene: a distinctive pattern of hypersensitive sites is characteristic for its transcriptional activity.The Biochemical journal · 1997Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The methylation patterns of the rat prolactin (rPRL) (positions -440 to -20) and growth hormone (rGH) (positions -360 to -110) promoters were analyzed by bisulfite genomic sequencing. Two normal tissues, the anterior pituitary and the liver, and three rat pituitary GH3 cell lines that differ considerably in their abilities to express both genes were tested. High levels of rPRL gene expression were correlated with hypomethylation of the CpG dinucleotides located at positions -277 and -97, near or within positive cis-acting regulatory elements. For the nine CpG sites analyzed in the rGH promoter, an overall hypomethylation-expression coupling was also observed for the anterior pituitary, the liver, and two of the cell lines. The effect of DNA methylation was tested by measuring the transient expression of the chloramphenicol acetyltransferase reporter gene driven by a regionally methylated rPRL promoter. CpG methylation resulted in a decrease in the activity of the rPRL promoter which was proportional to the number of modified CpG sites. The extent of the inhibition was also found to be dependent on the position of methylated sites. Taken together, these data suggest that site-specific methylation may modulate the action of transcription factors that dictate the tissue-specific expression of the rPRL and rGH genes in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.