ArticleMolecular and cellular biology1996
Yeast pip3/mec3 mutants fail to delay entry into S phase and to slow DNA replication in response to DNA damage, and they define a functional link between Mec3 and DNA primase.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 77 citations in OpenAlex.
- OsHUS1 facilitates accurate meiotic recombination in rice.PLoS genetics · 2014Article
- In vivo and in silico analysis of PCNA ubiquitylation in the activation of the Post Replication Repair pathway in S. cerevisiae.BMC systems biology · 2013Article
- A Rad53 independent function of Rad9 becomes crucial for genome maintenance in the absence of the Recq helicase Sgs1.PloS one · 2013Article
- G(1)/S and G(2)/M cyclin-dependent kinase activities commit cells to death in the absence of the S-phase checkpoint.Molecular and cellular biology · 2012Article
- Eukaryotic DNA damage checkpoint activation in response to double-strand breaks.Cellular and molecular life sciences : CMLS · 2012Review
- The Saccharomyces cerevisiae RAD9, RAD17 and RAD24 genes are required for suppression of mutagenic post-replicative repair during chronic DNA damage.DNA repair · 2010Article
- Dominant TEL1-hy mutations compensate for Mec1 lack of functions in the DNA damage response.Molecular and cellular biology · 2008Article
- A novel nonnull ZIP1 allele triggers meiotic arrest with synapsed chromosomes in Saccharomyces cerevisiae.Genetics · 2007Article
- Control of the yeast telomeric senescence survival pathways of recombination by the Mec1 and Mec3 DNA damage sensors and RPA.Nucleic acids research · 2007Article
- The functions of budding yeast Sae2 in the DNA damage response require Mec1- and Tel1-dependent phosphorylation.Molecular and cellular biology · 2004Article
- Physical and functional interactions between nucleotide excision repair and DNA damage checkpoint.The EMBO journal · 2004Article
- Functions of Saccharomyces cerevisiae 14-3-3 proteins in response to DNA damage and to DNA replication stress.Genetics · 2003Article
- Critical role for mouse Hus1 in an S-phase DNA damage cell cycle checkpoint.Molecular and cellular biology · 2003Article
- A dominant-negative MEC3 mutant uncovers new functions for the Rad17 complex and Tel1.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Role of the p68 subunit of human DNA polymerase alpha-primase in simian virus 40 DNA replication.Molecular and cellular biology · 2002Article
- Suppression of genome instability by redundant S-phase checkpoint pathways in Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Hyperactivation of the yeast DNA damage checkpoint by TEL1 and DDC2 overexpression.The EMBO journal · 2001Article
- Cdc13 prevents telomere uncapping and Rad50-dependent homologous recombination.The EMBO journal · 2001Article
- Topoisomerase III acts upstream of Rad53p in the S-phase DNA damage checkpoint.Molecular and cellular biology · 2001Article
- The set1Delta mutation unveils a novel signaling pathway relayed by the Rad53-dependent hyperphosphorylation of replication protein A that leads to transcriptional activation of repair genes.Genes & development · 2001Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The catalytic DNA primase subunit of the DNA polymerase alpha-primase complex is encoded by the essential PRI1 gene in Saccharomyces cerevisiae. To identify factors that functionally interact with yeast DNA primase in living cells, we developed a genetic screen for mutants that are lethal at the permissive temperature in a cold-sensitive pril-2 genetic background. Twenty-four recessive mutations belonging to seven complementation groups were identified. Some mutants showed additional phenotypes, such as increased sensitivity to UV irradiation, methyl methanesulfonate, and hydroxyurea, that were suggestive of defects in DNA repair and/or checkpoint mechanisms. We have cloned and characterized the gene of one complementation group, PIP3, whose product is necessary both for delaying entry into S phase or mitosis when cells are UV irradiated in G1 or G2 phase and for lowering the rate of ongoing DNA synthesis in the presence of methyl methanesulfonate. PIP3 turned out to be the MEC3 gene, previously identified as a component of the G2 DNA damage checkpoint. The finding that Mec3 is also required for the G1- and S-phase DNA damage checkpoints, together with the analysis of genetic interactions between a mec3 null allele and several conditional DNA replication mutations at the permissive temperature, suggests that Mec3 could be part of a mechanism coupling DNA replication with repair of DNA damage, and DNA primase might be involved in this process.
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