ArticleThe EMBO journal1996
An extradenticle-induced conformational change in a HOX protein overcomes an inhibitory function of the conserved hexapeptide motif.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
46 citing papers in PubMed, 117 citations in OpenAlex.
- Article
- Autoinhibited Protein Database: a curated database of autoinhibitory domains and their autoinhibition mechanisms.Database : the journal of biological databases and curation · 2024Article
- Innexin hemichannel activation byiScience · 2024Article
- Flexibility of flanking DNA is a key determinant of transcription factor affinity for the core motif.Biophysical journal · 2022Article
- The Hox protein conundrum: The "specifics" of DNA binding for Hox proteins and their partners.Developmental biology · 2021Review
- Anterior Hox Genes and the Process of Cephalization.Frontiers in cell and developmental biology · 2021Review
- A six-amino-acid motif is a major determinant in functional evolution of HOX1 proteins.Genes & development · 2020Article
- In vivo Hox binding specificity revealed by systematic changes to a single cis regulatory module.Nature communications · 2019Article
- Hindbrain induction and patterning during early vertebrate development.Cellular and molecular life sciences : CMLS · 2019Review
- Mechanisms of Specificity for Hox Factor Activity.Journal of developmental biology · 2016Article
- Inhibitory activities of short linear motifs underlie Hox interactome specificity in vivo.eLife · 2015Article
- The TALE transcription factor homothorax functions to assemble heterochromatin during Drosophila embryogenesis.PloS one · 2015Article
- Redox signaling in cardiac renewal.Antioxidants & redox signaling · 2014Review
- Homeotic functions of the Teashirt transcription factor during adult Drosophila development.Biology open · 2013Article
- Hox proteins coordinate peripodial decapentaplegic expression to direct adult head morphogenesis in Drosophila.Developmental biology · 2012Article
- Disentangling the many layers of eukaryotic transcriptional regulation.Annual review of genetics · 2012Review
- Hox proteins display a common and ancestral ability to diversify their interaction mode with the PBC class cofactors.PLoS biology · 2012Article
- Pbx homeodomain proteins: TALEnted regulators of limb patterning and outgrowth.Developmental dynamics : an official publication of the American Association of Anatomists · 2011Review
- Selection of distinct Hox-Extradenticle interaction modes fine-tunes Hox protein activity.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Comparing anterior and posterior Hox complex formation reveals guidelines for predicting cis-regulatory elements.Developmental biology · 2010Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
HOX homeoproteins control cell identities during animal development by differentially regulating target genes. The homeoprotein encoded by the extradenticle (exd) gene can selectively modify HOX DNA binding, suggesting that it contributes to HOX specificity in vivo. HOX-EXD interactions are in part mediated by a conserved stretch of amino acids termed the hexapeptide found in many HOX proteins. Here, we demonstrate that a 20 bp oligonucleotide from the 5' region of the mouse Hoxb-1 gene, a homolog of Drosophila labial (lab), is sufficient to direct an expression pattern in Drosophila that is very similar to endogenous lab. In vivo, this expression requires lab and exd and, in vitro, LAB requires EXD to bind this oligonucleotide. In contrast, LAB proteins with mutations in the hexapeptide bind DNA even in the absence of EXD. Moreover, a hexapeptide mutant of LAB has an increased ability to activate transcription in vivo. Partial proteolysis experiments suggest that EXD can induce a conformational change in LAB. These data are consistent with a mechanism whereby the LAB hexapeptide inhibits LAB function by inhibiting DNA binding and that an EXD-induced conformational change in LAB relieves this inhibition, promoting highly specific interactions with biologically relevant binding sites.
Indexed as
Identifiers
8665855PMC450180W214611139What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.