ArticlePflugers Archiv : European journal of physiology1996
The cAMP-regulated and 293B-inhibited K+ conductance of rat colonic crypt base cells.
Article in Pflugers Archiv : European journal of physiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 91 citations in OpenAlex.
- Kv7 Channels and Excitability Disorders.Handbook of experimental pharmacology · 2021Article
- Colonic Potassium Absorption and Secretion in Health and Disease.Comprehensive Physiology · 2018Review
- KThe Journal of physiology · 2018Article
- Upregulation of basolateral small conductance potassium channels (KCNQ1/KCNE3) in ulcerative colitis.Biochemical and biophysical research communications · 2016Article
- Cyclic AMP-induced K+ secretion occurs independently of Cl- secretion in rat distal colon.American journal of physiology. Cell physiology · 2012Article
- Sexual dimorphism and oestrogen regulation of KCNE3 expression modulates the functional properties of KCNQ1 K⁺ channels.The Journal of physiology · 2011Article
- AMP-activated protein kinase inhibits KCNQ1 channels through regulation of the ubiquitin ligase Nedd4-2 in renal epithelial cells.American journal of physiology. Renal physiology · 2010Article
- Disruption of the K+ channel beta-subunit KCNE3 reveals an important role in intestinal and tracheal Cl- transport.The Journal of biological chemistry · 2010Article
- Effects of chronic hypoxia on electrogenic transport and transport-related oxygen consumption in rat distal colon.Digestive diseases and sciences · 2008Article
- Segmental heterogeneity of electrogenic secretions in human ascending colon and rectum.International journal of colorectal disease · 2006Article
- KCNQ1-dependent transport in renal and gastrointestinal epithelia.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Effects of I(Ks) channel inhibitors in insulin-secreting INS-1 cells.Pflugers Archiv : European journal of physiology · 2005Article
- Class I antiarrhythmics inhibit Na+ absorption and Cl- secretion in rabbit descending colon epithelium.Naunyn-Schmiedeberg's archives of pharmacology · 2005Article
- Use of knock-out mouse models for the study of renal ion channels.The Journal of membrane biology · 2004Review
- Chloride secretion in a morphologically differentiated human colonic cell line that expresses the epithelial Na+ channel.The Journal of physiology · 2004Article
- Ca(2+) influx through the basolateral- and luminal membranes of colonic epithelium in neonatal rats.Pflugers Archiv : European journal of physiology · 2004Article
- Potassium channels in epithelial transport.Pflugers Archiv : European journal of physiology · 2003Review
- Swelling-activated chloride and potassium conductance in primary cultures of mouse proximal tubules. Implication of KCNE1 protein.The Journal of membrane biology · 2003Article
- Article
- Function of K+ channels in the intestinal epithelium.The Journal of membrane biology · 2003Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have shown previously that secretagogues acting via the second messenger adenosine 3',5'-cyclic monophosphate (cAMP) activate, besides their marked effect on the luminal Cl- conductance, a K+ conductance in the basolateral membrane of colonic crypt cells. This conductance is blocked by the chromanol 293B. This K+ conductance is examined here in more detail in cell-attached (c.a.) and cell-excised (c.e.) patch- clamp studies. Addition of forskolin (5 micromol/l) to the bath led to the activation of very small-conductance (probably < 3 pS) K+ channels in c.a. patches (n = 54). These channels were reversibly inhibited by the addition of 0.1 mmol/l of 293B to the bath (n = 21). Noise analysis revealed that these channels had fast kinetics and produced a Lorentzian noise component with a corner frequency (fc) of 308 +/- 10 Hz (n = 30). The current/voltage curves of this noise indicated that the underlying ion channels were K+ selective. 293B reduced the power density of the noise (So) to 46 +/- 8.7% of its control value and shifted fc from 291 +/- 26 to 468 +/- 54 Hz (n = 8). In c.e. patches from cells previously stimulated by forskolin, the same type of current persisted in 3 out of 18 experiments when the bath solution was a cytosolic-type solution without adenosine 5'-triphosphate (ATP) (CYT). In 15 experiments the addition of ATP (1 mmol/l) to CYT solution was necessary to induce or augment channel activity. In six experiments excision was performed into CYT + ATP solution and channel activity persisted. 293B exerted a reversible inhibitory effect. The channel activity was reduced by 5 mmol/l Ba2+ and was completely absent when K+ in the bath was replaced by Na+. These data suggest that forskolin activates a K+ channel of very small conductance which can be inhibited directly and reversibly by 293B.
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