ArticleCurrent genetics1996
Molecular and functional characterization of a mutant allele of the mitogen-activated protein-kinase gene SLT2(MPK1) rescued from yeast autolytic mutants.
Article in Current genetics, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 52 citations in OpenAlex.
- The Yeast Gsk-3 Kinase Mck1 Is Necessary for Cell Wall Remodeling in Glucose-Starved and Cell Wall-Stressed Cells.International journal of molecular sciences · 2025Article
- A walk-through MAPK structure and functionality with the 30-year-old yeast MAPK Slt2.International microbiology : the official journal of the Spanish Society for Microbiology · 2021Review
- Global analysis of Saccharomyces cerevisiae growth in mucin.G3 (Bethesda, Md.) · 2021Article
- SSD1 suppresses phenotypes induced by the lack of Elongator-dependent tRNA modifications.PLoS genetics · 2019Article
- Cell wall perturbation sensitizes fungi to the antimalarial drug chloroquine.Antimicrobial agents and chemotherapy · 2013Article
- Functional wiring of the yeast kinome revealed by global analysis of genetic network motifs.Genome research · 2012Article
- The many faces of mitochondrial autophagy: making sense of contrasting observations in recent research.International journal of cell biology · 2012Article
- Use of the plant defense protein osmotin to identify Fusarium oxysporum genes that control cell wall properties.Eukaryotic cell · 2010Article
- Boric Acid Disturbs Cell Wall Synthesis in Saccharomyces cerevisiae.International journal of microbiology · 2010Article
- Mechanism of Mpk1 mitogen-activated protein kinase binding to the Swi4 transcription factor and its regulation by a novel caffeine-induced phosphorylation.Molecular and cellular biology · 2009Article
- New mutant versions of yeast FACT subunit Spt16 affect cell integrity.Molecular genetics and genomics : MGG · 2009Article
- Isolation of intrinsically active (MEK-independent) variants of the ERK family of mitogen-activated protein (MAP) kinases.The Journal of biological chemistry · 2008Article
- Medicago LYK3, an entry receptor in rhizobial nodulation factor signaling.Plant physiology · 2007Article
- The Cdc34/SCF ubiquitination complex mediates Saccharomyces cerevisiae cell wall integrity.Genetics · 2006Article
- The Sho1 adaptor protein links oxidative stress to morphogenesis and cell wall biosynthesis in the fungal pathogen Candida albicans.Molecular and cellular biology · 2005Article
- Osmotic stress signaling and osmoadaptation in yeasts.Microbiology and molecular biology reviews : MMBR · 2002Review
- Article
- A genomic approach for the identification and classification of genes involved in cell wall formation and its regulation in Saccharomyces cerevisiae.Comparative and functional genomics · 2001Article
- A complex containing RNA polymerase II, Paf1p, Cdc73p, Hpr1p, and Ccr4p plays a role in protein kinase C signaling.Molecular and cellular biology · 1999Article
- MAP kinase pathways in the yeast Saccharomyces cerevisiae.Microbiology and molecular biology reviews : MMBR · 1998Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have further characterized the functionality of the Saccharomyces cerevisiae gene SLT2(MPK1), coding for a MAP-kinase homolog essential for cell integrity, which is involved in the Pkc1p signalling pathway. This gene was isolated on the basis of its capacity to complement the thermosensitive-autolytic, osmotic-remediable phenotype of lyt2 mutants. Both slt2delta and lyt2 mutants displayed a caffeine-sensitive phenotype consisting of cell lysis that was not dependent on temperature. Caffeine concentrations affecting the growth of these mutant strains were dependent on the genetic background, the SSD1 allele being very significant in this regard. The SLT2 allele of several lyt2 strains was both rescued and amplified by PCR. The recovered allele was shown to be non-functional as it could not complement the lytic phenotype of both deletion (slt2delta) and lyt2 strains. After nucleotide sequencing of the recovered allele, we found that the defect of lyt2 mutants consists in a substitution of an aspartic acid for a glycine at position 35 of the amino-acid sequence of Slt2p. Gly35 is the third glycine of a glycine cluster (Gly-X-Gly-X-X-Gly), a conserved region in protein kinases and other nucleotide-binding proteins. Keywords Yeast middle dot SLT2 middle dot MAP-kinase middle dot Caffeine
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