ArticleThe Biochemical journal1996
Extracellular calcium concentration controls the frequency of intracellular calcium spiking independently of inositol 1,4,5-trisphosphate production in HeLa cells.
Article in The Biochemical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 75 citations in OpenAlex.
- Fundamentals of Cellular Calcium Signaling: A Primer.Cold Spring Harbor perspectives in biology · 2020Review
- Photoswitchable diacylglycerols enable optical control of protein kinase C.Nature chemical biology · 2016Article
- Differential Regulation of Multiple Steps in Inositol 1,4,5-Trisphosphate Signaling by Protein Kinase C Shapes Hormone-stimulated Ca2+ Oscillations.The Journal of biological chemistry · 2015Article
- Regulation of oscillation dynamics in biochemical systems with dual negative feedback loops.Journal of the Royal Society, Interface · 2012Article
- Orai3--the 'exceptional' Orai?The Journal of physiology · 2012Review
- Calpain-1 cleaves Rad21 to promote sister chromatid separation.Molecular and cellular biology · 2011Article
- Orai channel-dependent activation of phospholipase C-δ: a novel mechanism for the effects of calcium entry on calcium oscillations.The Journal of physiology · 2011Article
- Arachidonic acid, ARC channels, and Orai proteins.Cell calcium · 2009Review
- Calcium influx mechanisms underlying calcium oscillations in rat hepatocytes.Hepatology (Baltimore, Md.) · 2008Article
- Modulation of Ca(2+) release and Ca(2+) oscillations in HeLa cells and fibroblasts by mitochondrial Ca(2+) uniporter stimulation.The Journal of physiology · 2007Article
- Origin of spontaneous rhythmicity in smooth muscle.The Journal of physiology · 2006Review
- Calcium oscillations in interstitial cells of the rabbit urethra.The Journal of physiology · 2005Article
- The frequencies of calcium oscillations are optimized for efficient calcium-mediated activation of Ras and the ERK/MAPK cascade.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Control of calcium oscillations by membrane fluxes.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Control analysis for autonomously oscillating biochemical networks.Biophysical journal · 2002Article
- Stimulus-dependent control of inositol 1,4,5-trisphosphate-induced Ca(2+) oscillation frequency by the endoplasmic reticulum Ca(2+)-ATPase.Biophysical journal · 2001Article
- Single-cell imaging of graded Ins(1,4,5)P3 production following G-protein-coupled-receptor activation.The Biochemical journal · 2001Article
- Effects of elevated expression of inositol 1,4,5-trisphosphate 3-kinase B on Ca2+ homoeostasis in HeLa cells.The Biochemical journal · 2000Article
- Receptor-activated Ca2+ inflow in animal cells: a variety of pathways tailored to meet different intracellular Ca2+ signalling requirements.The Biochemical journal · 1999Review
- Ca2+ influx does more than provide releasable Ca2+ to maintain repetitive spiking in human umbilical vein endothelial cells.The Biochemical journal · 1996Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stimulation of single HeLa cells with histamine evoked repetitive increases of the intracellular calcium ion concentration (Ca2+ spikes). The frequency of Ca2+ spiking increased as the extracellular hormone concentration was elevated. In addition, the frequency of Ca2+ spiking could be accelerated by increasing the extracellular Ca2+ concentration ([Ca2+]0) in the presence of a constant hormone concentration. The range of [Ca2+]0 over which the spiking frequency could be titrated was nominally-zero to 10mM, being half-maximally effective at approx. 1 and 2.5mM for 37 and 22 degrees C respectively. The effect of [Ca2+]0 on inositol phosphates production was also examined. Changes of [Ca2+]0 over a range which had been found to affect the frequency of Ca2+ spiking did not have any effect on the rate of myo-inositol 1,4,5-trisphosphate (InsP3) production, although an increase in inositol phosphates production was observed as [Ca2+]0 was increased from zero to values giving less than half-maximal Ca2+ spike frequency. These data suggest that at low Ca2+ spike frequency, Ca2+-stimulated activation of phospholipase C may contribute to Ca2+ spiking in HeLa cells, but under some conditions the availability of Ca2+ to the intracellular stores, rather than changes in the rate of InsP3 production, determines the Ca2+ spike frequency.
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