ArticleNucleic acids research1996
Mutagenicity of a unique thymine-thymine dimer or thymine-thymine pyrimidine pyrimidone (6-4) photoproduct in mammalian cells.
Article in Nucleic acids research, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 78 citations in OpenAlex.
- A Comprehensive Assessment of Ultraviolet-Radiation-Induced Mutations inJournal of fungi (Basel, Switzerland) · 2024Article
- Transcription and DNA repair collide after UV exposure.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Establishing Linkages Among DNA Damage, Mutagenesis, and Genetic Diseases.Chemical research in toxicology · 2022Review
- Mechanisms of UV-induced mutations and skin cancer.Genome instability & disease · 2020Article
- Mechanisms of DNA damage, repair, and mutagenesis.Environmental and molecular mutagenesis · 2017Review
- Conformational polymorphism or structural invariance in DNA photoinduced lesions: implications for repair rates.Nucleic acids research · 2017Article
- DNA sequence context greatly affects the accuracy of bypass across an ultraviolet light 6-4 photoproduct in mammalian cells.Mutation research · 2015Article
- Response of Dictyostelium discoideum to UV-C and involvement of poly (ADP-ribose) polymerase.Cell proliferation · 2015Article
- The structure of an authentic spore photoproduct lesion in DNA suggests a basis for recognition.Acta crystallographica. Section D, Biological crystallography · 2014Article
- Genomic assay reveals tolerance of DNA damage by both translesion DNA synthesis and homology-dependent repair in mammalian cells.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- UV wavelength-dependent DNA damage and human non-melanoma and melanoma skin cancer.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2012Review
- DNA damage bypass operates in the S and G2 phases of the cell cycle and exhibits differential mutagenicity.Nucleic acids research · 2012Article
- Genetic requirement for mutagenesis of the G[8,5-Me]T cross-link in Escherichia coli: DNA polymerases IV and V compete for error-prone bypass.Biochemistry · 2011Article
- Article
- Article
- Human DNA polymerase kappa synthesizes DNA with extraordinarily low fidelity.Nucleic acids research · 2000Article
- The (6-4) photoproduct of thymine-thymine induces targeted substitution mutations in mammalian cells.Nucleic acids research · 1998Article
- Antigen structural requirements for recognition by a cyclobutane thymine dimer-specific monoclonal antibody.Nucleic acids research · 1997Article
- Recognition and repair of compound DNA lesions (base damage and mismatch) by human mismatch repair and excision repair systems.Molecular and cellular biology · 1997Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The mutagenic properties of UV-induced photoproducts, both the cis-syn thymine-thymine dimer (TT) and the thymine-thymine pyrimidine pyrimidone (6-4) photoproduct [T(6-4)T] were studied in mammalian cells using shuttle vectors. A shuttle vector able to replicate in both mammalian cells and bacteria was produced in its single-stranded DNA form. A unique photoproduct was inserted at a single restriction site and after recircularization of the single-stranded DNA vector, this latter was transfected into simian COS7 cells. After DNA replication the vector was extracted from cells and used to transform bacteria. Amplified DNA was finally analyzed without any selective screening, DNA from randomly picked bacterial colonies being directly sequenced. Our results show clearly that both lesions are mutagenic, but at different levels. Mutation frequencies of 2 and 60% respectively were observed with the TT dimer and the T(6-4)T. With the TT dimer the mutations were targeted on the 3'-T. With the T(6-4)T a large variety of mutations were observed. A majority of G-->T transversions were semi-targeted to the base before the 5'-T of the photoproduct. These kinds of mutations were not observed when the same plasmid was transfected directly into SOS-induced JM105 bacteria or when the T(6-4)T oligonucleotide inserted in a different plasmid was replicated in SOS-induced SMH10 Escherichia coil bacteria. These semi-targeted mutations are therefore the specific result of bypass of the T(6-4)T lesion in COS7 cells by one of the eukaryotic DNA polymerases.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.