ArticleMolecular and cellular biology1996
Topoisomerase I involvement in illegitimate recombination in Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 91 citations in OpenAlex.
- Patch type nucleotide sequence identities between genomes from many different species facilitate illegitimate recombination.Scientific reports · 2026Article
- The Top1 paradox: Friend and foe of the eukaryotic genome.DNA repair · 2017Review
- Topoisomerase-I PS506 as a Dual Function Cancer Biomarker.PloS one · 2015Article
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- Effect of rad50 mutation on illegitimate recombination in Saccharomyces cerevisiae.Molecular genetics and genomics : MGG · 2011Article
- Ty1 integrase overexpression leads to integration of non-Ty1 DNA fragments into the genome of Saccharomyces cerevisiae.Molecular genetics and genomics : MGG · 2010Review
- Mechanism and specificity of DNA strand exchange catalyzed by vaccinia DNA topoisomerase type I.Biochemistry · 2010Article
- Duplications involving a conserved regulatory element downstream of BMP2 are associated with brachydactyly type A2.American journal of human genetics · 2009Article
- Article
- Sequence features of HLA-DRB1 locus define putative basis for gene conversion and point mutations.BMC genomics · 2008Article
- Ionizing radiation and restriction enzymes induce microhomology-mediated illegitimate recombination in Saccharomyces cerevisiae.Nucleic acids research · 2007Article
- Promoter shuffling at a nuclear gene for mitochondrial RPL27. Involvement of interchromosome and subsequent intrachromosome recombinations.Plant physiology · 2006Article
- Origination of an X-linked testes chimeric gene by illegitimate recombination in Drosophila.PLoS genetics · 2006Article
- Poly(ADP-RIBOSE) polymerase-1 (Parp-1) antagonizes topoisomerase I-dependent recombination stimulation by P53.Nucleic acids research · 2006Article
- Genome wide distribution of illegitimate recombination events in Kluyveromyces lactis.Nucleic acids research · 2006Article
- Genomic rearrangements at the IGHMBP2 gene locus in two patients with SMARD1.Human genetics · 2004Article
- Human topoisomerase I mediates illegitimate recombination leading to DNA insertion into the ribosomal DNA locus in Saccharomyces cerevisiae.Molecular genetics and genomics : MGG · 2004Article
- Human topoisomerase I cleavage complexes are repaired by a p53-stimulated recombination-like reaction in vitro.Nucleic acids research · 2002Article
- Subnuclear distribution of topoisomerase I is linked to ongoing transcription and p53 status.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chromosome aberrations may cause cancer and many heritable diseases. Topoisomerase I has been suspected of causing chromosome aberrations by mediating illegitimate recombination. The effects of deletion and of overexpression of the topoisomerase I gene on illegitimate recombination in the yeast Saccharomyces cerevisiae have been studied. Yeast transformations were carried out with DNA fragments that did not have any homology to the genomic DNA. The frequency of illegitimate integration was 6- to 12-fold increased in a strain overexpressing topoisomerase I compared with that in isogenic control strains. Hot spot sequences [(G/C)(A/T)T] for illegitimate integration target sites accounted for the majority of the additional events after overexpression of topoisomerase I. These hot spot sequences correspond to sequences previously identified in vitro as topoisomerase I preferred cleavage sequences in other organisms. Furthermore, such hot spot sequences were found in 44% of the integration events present in the TOP1 wild-type strain and at a significantly lower frequency in the top1delta strain. Our results provide in vivo evidence that a general eukaryotic topoisomerase I enzyme nicks DNA and ligates nonhomologous ends, leading to illegitimate recombination.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.