ArticleMolecular and cellular biology1996
Isolation and characterization of the cDNA encoding BKLF/TEF-2, a major CACCC-box-binding protein in erythroid cells and selected other cells.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers.
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Who cites it
84 citing papers in PubMed, 226 citations in OpenAlex.
- Function ofOncology letters · 2025Review
- WDR5 serves in co-activation and influences genome targeting of KLF3.Nucleic acids research · 2025Article
- Erythroid Krüppel-Like Factor (KLF1): A Surprisingly Versatile Regulator of Erythroid Differentiation.Advances in experimental medicine and biology · 2024Review
- Protocols for protein-DNA binding analysis of a zinc finger transcription factor bound to its cognate promoter.STAR protocols · 2022Article
- Article
- Structural basis for human ZBTB7A action at the fetal globin promoter.Cell reports · 2021Article
- Article
- ZNF410 Uniquely Activates the NuRD Component CHD4 to Silence Fetal Hemoglobin Expression.Molecular cell · 2021Article
- Article
- Methylation of a CGATA element inhibits binding and regulation by GATA-1.Nature communications · 2020Article
- Krüppel-like factor 3 (KLF3) suppresses NF-κB-driven inflammation in mice.The Journal of biological chemistry · 2020Article
- Expression of human Krüppel-like factor 3 in peripheral blood as a promising biomarker for acute leukemia.Cancer medicine · 2020Article
- Systematic genome editing of the genes on zebrafish Chromosome 1 by CRISPR/Cas9.Genome research · 2019Article
- MiR-326/Sp1/KLF3: A novel regulatory axis in lung cancer progression.Cell proliferation · 2019Article
- Article
- Review
- Natural regulatory mutations elevate the fetal globin gene via disruption of BCL11A or ZBTB7A binding.Nature genetics · 2018Article
- Krüppel-like factors: Crippling and un-crippling metabolic pathways.JACC. Basic to translational science · 2018Article
- Krüppel-like factors compete for promoters and enhancers to fine-tune transcription.Nucleic acids research · 2017Article
- Direct competition between DNA binding factors highlights the role of Krüppel-like Factor 1 in the erythroid/megakaryocyte switch.Scientific reports · 2017Article
24 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CACCC boxes are among the critical sequences present in regulatory elements of genes expressed in erythroid cells, as well as in selected other cell types. While an erythroid cell-specific CACCC-box-binding protein, EKLF, has been shown to be required in vivo for proper expression of the adult beta-globin gene, it is dispensable for the regulation of several other globin and nonglobin erythroid cell-expressed genes. In the work described here, we searched for additional CACCC-box transcription factors that might be active in murine erythroid cells. We identified a major gel shift activity (termed BKLF), present in yolk sac and fetal liver erythroid cells, that could be distinguished from EKLF by specific antisera. Through relaxed-stringency hybridization, we obtained the cDNA encoding BKLF, a highly basic, novel zinc finger protein that is related to EKLF and other Krüppel-like members in its DNA-binding domain but unrelated elsewhere. BKLF, which is widely but not ubiquitously expressed in cell lines, is highly expressed in the midbrain region of embryonic mice and appears to correspond to the gel shift activity TEF-2, a transcriptional activator implicated in regulation of the simian virus 40 enhancer and other CACCC-box-containing regulatory elements. Because BKLF binds with high affinity and preferentially over Sp1 to many CACCC sequences of erythroid cell expressed genes, it is likely to participate in the control of many genes whose expression appears independent of the action of EKLF.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.