ArticleMolecular and cellular biology1996
Analysis of muscle creatine kinase gene regulatory elements in skeletal and cardiac muscles of transgenic mice.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed, 95 citations in OpenAlex.
- Have a Little Heart (or Not): Highly Minimized Skeletal Muscle Regulatory Cassettes with Low or No Activity in the Heart.Human gene therapy · 2024Article
- Construction of a Porcine Skeletal Muscle-Specific Promoter by Inducing the Seed Region of miR-208a.Molecular biotechnology · 2022Article
- Targeted epigenetic repression by CRISPR/dSaCas9 suppresses pathogenicMolecular therapy. Methods & clinical development · 2021Article
- FGF21 underlies a hormetic response to metabolic stress in methylmalonic acidemia.JCI insight · 2018Article
- Incorporation of a skeletal muscle-specific enhancer in the regulatory region of Igf1 upregulates IGF1 expression and induces skeletal muscle hypertrophy.Scientific reports · 2018Article
- Article
- Enhanced fasting glucose turnover in mice with disrupted action of TUG protein in skeletal muscle.The Journal of biological chemistry · 2013Article
- Upregulation of triglyceride synthesis in skeletal muscle overexpressing DGAT1.Lipids in health and disease · 2013Article
- Krüppel-like factor 6 (KLF6) promotes cell proliferation in skeletal myoblasts in response to TGFβ/Smad3 signaling.Skeletal muscle · 2013Article
- Interrogating transcriptional regulatory sequences in Tol2-mediated Xenopus transgenics.PloS one · 2013Article
- YY1 negatively regulates mouse myelin proteolipid protein (Plp1) gene expression in oligodendroglial cells.ASN neuro · 2011Article
- Estrogen-related receptor α regulates skeletal myocyte differentiation via modulation of the ERK MAP kinase pathway.American journal of physiology. Cell physiology · 2011Article
- Mice lacking microRNA 133a develop dynamin 2–dependent centronuclear myopathy.The Journal of clinical investigation · 2011Article
- Differentiation and fiber type-specific activity of a muscle creatine kinase intronic enhancer.Skeletal muscle · 2011Article
- Identification of differentially regulated secretome components during skeletal myogenesis.Molecular & cellular proteomics : MCP · 2011Article
- Sequential association of myogenic regulatory factors and E proteins at muscle-specific genes.Skeletal muscle · 2011Article
- KLF3 regulates muscle-specific gene expression and synergizes with serum response factor on KLF binding sites.Molecular and cellular biology · 2010Article
- TEAD-1 overexpression in the mouse heart promotes an age-dependent heart dysfunction.The Journal of biological chemistry · 2010Article
- Nuclear function of Smad7 promotes myogenesis.Molecular and cellular biology · 2010Article
- Cardiotrophin-1 maintains the undifferentiated state in skeletal myoblasts.The Journal of biological chemistry · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 3 countries.
Funding
Abstract
Regulatory regions of the mouse muscle creatine kinase (MCK) gene, previously discovered by analysis in cultured muscle cells, were analyzed in transgenic mice. The 206-bp MCK enhancer at nt-1256 was required for high-level expression of MCK-chloramphenicol acetyltransferase fusion genes in skeletal and cardiac muscle; however, unlike its behavior in cell culture, inclusion of the 1-kb region of DNA between the enhancer and the basal promoter produced a 100-fold increase in skeletal muscle activity. Analysis of enhancer control elements also indicated major differences between their properties in transgenic muscles and in cultured muscle cells. Transgenes in which the enhancer right E box or CArG element were mutated exhibited expression levels that were indistinguishable from the wild-type transgene. Mutation of three conserved E boxes in the MCK 1,256-bp 5' region also had no effect on transgene expression in thigh skeletal muscle expression. All these mutations significantly reduced activity in cultured skeletal myocytes. However, the enhancer AT-rich element at nt - 1195 was critical for expression in transgenic skeletal muscle. Mutation of this site reduced skeletal muscle expression to the same level as transgenes lacking the 206-bp enhancer, although mutation of the AT-rich site did not affect cardiac muscle expression. These results demonstrate clear differences between the activity of MCK regulatory regions in cultured muscles cells and in whole adult transgenic muscle. This suggests that there are alternative mechanism of regulating the MCK gene in skeletal and cardiac muscle under different physiological states.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.