ArticleMolecular and cellular biology1996
Ku86 defines the genetic defect and restores X-ray resistance and V(D)J recombination to complementation group 5 hamster cell mutants.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 152 citations in OpenAlex.
- Cytoplasmic-translocated Ku70 senses intracellular DNA and mediates interferon-lambda1 induction.Immunology · 2021Article
- (5'International journal of molecular sciences · 2021Article
- Diminished or inversed dose-rate effect on clonogenic ability in Ku-deficient rodent cells.Journal of radiation research · 2021Article
- Inhibition of the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs) stimulates osteoblastogenesis by potentiating bone morphogenetic protein 2 (BMP2) responses.Journal of cellular physiology · 2021Article
- The Role of Ku70 as a Cytosolic DNA Sensor in Innate Immunity and Beyond.Frontiers in cellular and infection microbiology · 2021Review
- Ubiquitylation of Ku80 by RNF126 Promotes Completion of Nonhomologous End Joining-Mediated DNA Repair.Molecular and cellular biology · 2017Article
- Influenza infection induces host DNA damage and dynamic DNA damage responses during tissue regeneration.Cellular and molecular life sciences : CMLS · 2015Article
- BRCA2 is needed for both repair and cell cycle arrest in mammalian cells exposed to S23906, an anticancer monofunctional DNA binder.Cell cycle (Georgetown, Tex.) · 2015Article
- TRF2 interaction with Ku heterotetramerization interface gives insight into c-NHEJ prevention at human telomeres.Cell reports · 2013Article
- Spatiotemporal dynamics of DNA repair proteins following laser microbeam induced DNA damage - when is a DSB not a DSB?Mutation research · 2013Review
- Dual functions of the homeoprotein DLX4 in modulating responsiveness of tumor cells to topoisomerase II-targeting drugs.Cancer research · 2013Article
- A mechanism for the inhibition of DNA-PK-mediated DNA sensing by a virus.PLoS pathogens · 2013Article
- Impact of the Ku complex on HIV-1 expression and latency.PloS one · 2013Article
- The dynamics of Ku70/80 and DNA-PKcs at DSBs induced by ionizing radiation is dependent on the complexity of damage.Nucleic acids research · 2012Article
- Computational studies on full-length Ku70 with DNA duplexes: base interactions and a helical path.Journal of molecular modeling · 2012Article
- The DNA repair complex Ku70/86 modulates Apaf1 expression upon DNA damage.Cell death and differentiation · 2011Article
- Response to multiple radiation doses of human colorectal carcinoma cells infected with recombinant adenovirus containing dominant-negative Ku70 fragment.International journal of radiation oncology, biology, physics · 2010Article
- Activation of PARP-1 in response to bleomycin depends on the Ku antigen and protein phosphatase 5.Oncogene · 2010Article
- Ku regulates the non-homologous end joining pathway choice of DNA double-strand break repair in human somatic cells.PLoS genetics · 2010Article
- The nanny model for IDPs.Nature chemical biology · 2009Article
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
Abstract
X-ray-sensitive hamster cells in complementation groups 4, 5, 6, and 7 are impaired for both double-strand break repair and V(D)J recombination. Here we show that in two mutant cell lines (XR-V15B and XR-V9B) from group 5, the genetic defects are in the gene encoding the 86-kDa subunit of the Ku autoantigen, a nuclear protein that binds to the double-stranded DNA ends. These mutants express Ku86 mRNA containing deletions of 138 and 252 bp, respectively, and the encoded proteins contain internal, in-frame deletions of 46 and 84 amino acids. Two X-ray-resistant revertants of XR-V15B expressed two Ku86 transcripts, one with and one without the deletion, suggesting that reversion occurred by activation of a silent wild-type allele. Transfection of full-length cDNA encoding hamster Ku86 into XR-V15B cells resulted in a complete rescue of DNA-end-binding (DEB) activity and Ku70 levels, suggesting that Ku86 stabilizes the Ku70 polypeptide. In addition, cells expressing wild-type levels of DEB activity were fully rescued for X-ray resistance and V(D)J recombination, whereas cells expressing lower levels of DEB activity were only partially rescued. Thus, Ku is an essential component of the pathway(s) utilized for the resolution of DNA double-strand breaks induced by either X rays or V(D)J recombination, and mutations in the Ku86 gene are responsible for the phenotype of group 5 cells.
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