ArticleMolecular and cellular biology1996
Identification of two RNA-binding proteins in Balbiani ring premessenger ribonucleoprotein granules and presence of these proteins in specific subsets of heterogeneous nuclear ribonucleoprotein particles.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 49 citations in OpenAlex.
- Intranuclear binding in space and time of exon junction complex and NXF1 to premRNPs/mRNPs in vivo.The Journal of cell biology · 2015Article
- The Chironomus tentans genome sequence and the organization of the Balbiani ring genes.BMC genomics · 2014Article
- A cylindrical zoom lens unit for adjustable optical sectioning in light sheet microscopy.Biomedical optics express · 2010Article
- Article
- SWI/SNF associates with nascent pre-mRNPs and regulates alternative pre-mRNA processing.PLoS genetics · 2009Article
- A specific SR protein binds preferentially to the secretory protein gene transcripts in salivary glands of Chironomus tentans.Chromosoma · 2006Article
- Chironomus tentans-repressor splicing factor represses SR protein function locally on pre-mRNA exons and is displaced at correct splice sites.Molecular biology of the cell · 2006Article
- Hrp59, an hnRNP M protein in Chironomus and Drosophila, binds to exonic splicing enhancers and is required for expression of a subset of mRNAs.The Journal of cell biology · 2005Article
- Conspicuous accumulation of transcription elongation repressor hrp130/CA150 on the intron-rich Balbiani ring 3 gene.Chromosoma · 2004Article
- An actin-ribonucleoprotein interaction is involved in transcription by RNA polymerase II.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- A novel conserved RNA-binding domain protein, RBD-1, is essential for ribosome biogenesis.Molecular biology of the cell · 2002Article
- PSF and p54nrb bind a conserved stem in U5 snRNA.RNA (New York, N.Y.) · 2002Article
- Evidence for a posttranscriptional role of a TFIIICalpha-like protein in Chironomus tentans.Molecular biology of the cell · 2002Article
- Nuclear actin is associated with a specific subset of hnRNP A/B-type proteins.Nucleic acids research · 2002Article
- Distinct RNP complexes of shuttling hnRNP proteins with pre-mRNA and mRNA: candidate intermediates in formation and export of mRNA.Molecular and cellular biology · 2001Article
- Assembly and transport of a premessenger RNP particle.Proceedings of the National Academy of Sciences of the United States of America · 2001Review
- Actin bound to the heterogeneous nuclear ribonucleoprotein hrp36 is associated with Balbiani ring mRNA from the gene to polysomes.The Journal of cell biology · 2001Article
- The Ct-RAE1 protein interacts with Balbiani ring RNP particles at the nuclear pore.RNA (New York, N.Y.) · 2000Article
- Electron tomography reveals posttranscriptional binding of pre-mRNPs to specific fibers in the nucleoplasm.The Journal of cell biology · 2000Article
- Modulation of exon skipping by high-affinity hnRNP A1-binding sites and by intron elements that repress splice site utilization.The EMBO journal · 1999Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Balbiani ring (BR) granules are premessenger ribonucleoprotein particles (RNPs) generated in giant chromosomal puffs, the BRs, in the larval salivary glands of the dipteran chironomus tentans. Monoclonal antibodies were raised against nuclear proteins collected on a single-stranded-DNA-agarose affinity column, and two of them were used to identify RNA-binding proteins in BR granules. First, in Western blots (immunoblots), one of the antibodies recognized a 36-kDa protein and the other recognized a 45-KDa protein. Second, both antibodies bound to the BRs in immunocytological experiments. It was shown in cross-linking experiments that the two proteins are associated with heterogeneous nuclear RNP (hnRNP) complexes extracted from C. tentans nuclei. By immunoelectron microscopy of isolated and partly unfolded BR RNPs, it was specifically demonstrated that the BR granules contain the two proteins and, in addition, that both proteins are distributed frequently along the RNP fiber of the particles. Thus, the 36- and 45-KDa proteins are likely to be abundant, RNA-binding proteins in the BR particles. To elucidate to what extent the two proteins are also present in other hnRNPs, we studied the binding of the antibodies to chromosomal puffs in general. It was observed that many puffs in addition to the BRs harbor the two proteins, but there are also puffs containing only one of the components, either the 36- or the 45-kDa protein. We conclude that the two proteins are not randomly bound to all hnRNPs but that each of them seems to be linked to a specific subset of the particles.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.