Trial reportJournal of clinical pathology1996
Single-tube nested PCR in the diagnosis of tuberculosis.
Trial report in Journal of clinical pathology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 57 citations in OpenAlex.
- Nucleic acid amplification tests in the diagnosis of tuberculous pleuritis: a systematic review and meta-analysis.BMC infectious diseases · 2004Pooled it
- Clinical utility of target amplicon sequencing test for rapid diagnosis of drug-resistantFrontiers in microbiology · 2022Article
- Development of a two-step nucleic acid amplification test for accurate diagnosis of the Mycobacterium tuberculosis complex.Scientific reports · 2021Article
- Mycobacterium tuberculosis infection is associated with the development of erythema nodosum and nodular vasculitis.PloS one · 2013Article
- The Diagnostic Utility of Bact/ALERT and Nested PCR in the Diagnosis of Tuberculous Meningitis.Journal of clinical and diagnostic research : JCDR · 2013Article
- Novel pan-genomic analysis approach in target selection for multiplex PCR identification and detection of Burkholderia pseudomallei, Burkholderia thailandensis, and Burkholderia cepacia complex species: a proof-of-concept study.Journal of clinical microbiology · 2011Article
- Molecular characterization of fluoroquinolone resistance in Mycobacterium tuberculosis: functional analysis of gyrA mutation at position 74.Antimicrobial agents and chemotherapy · 2011Article
- Sex- and age-dependent association of SLC11A1 polymorphisms with tuberculosis in Chinese: a case control study.BMC infectious diseases · 2007Article
- Evaluation of polymerase chain reaction and adenosine deaminase assay for the diagnosis of tuberculous effusions in children.Archives of disease in childhood · 2006Article
- Direct detection of rifampin-resistant mycobacterium tuberculosis in respiratory specimens by PCR-DNA sequencing.Journal of clinical microbiology · 2004Article
- Clinical evaluation of the polymerase chain reaction for the rapid diagnosis of tuberculosis.Journal of clinical pathology · 2004Article
- Single-tube balanced heminested PCR for detecting Mycobacterium tuberculosis in smear-negative samples.Journal of clinical microbiology · 2000Article
- PCR based detection of mycobacteria in paraffin wax embedded material routinely processed for morphological examination.Molecular pathology : MP · 1999Article
- Evaluation of three commercial detection systems for Mycobacterium tuberculosis where clinical diagnosis is difficult.Journal of clinical pathology · 1999Article
- Peritoneal tuberculosis: diagnostic options.Infectious diseases in obstetrics and gynecology · 1999Article
- Comparison of two automated DNA amplification systems with a manual one-tube nested PCR assay for diagnosis of pulmonary tuberculosis.Journal of clinical microbiology · 1997Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimsTo evaluate the usefulness of a single-tube nested polymerase chain reaction (PCR) assay in the diagnosis of tuberculosis in 1497 pulmonary and 536 extrapulmonary specimens.
methodsA single-tube nested PCR, utilising two sets of primers with different melting temperatures (88 degrees C for external primers; 70 degrees C for internal primers) to augment sensitivity and specificity without increasing the risk of amplicon contamination, was evaluated. Specimens were initially tested for the repetitive IS6110 sequences and if negative, retested for the universal 38 kilodalton sequence and for inhibitors. dUTP/Uracil-N-glycosylase and Instagene treatment were used to minimise contamination and the effect of inhibitors, respectively.
resultsUsing culture as the gold standard, the overall sensitivity of the assay was 89% for pulmonary and 42% for extrapulmonary specimens. Sensitivity varied greatly with respect to sample type (92% for follow up specimens from a chest hospital and 70% for non-follow up specimens from a general hospital). The smear positivity rates were 15% for extrapulmonary specimens, and 69% and 45%, respectively, for follow up and non-follow up specimens from pulmonary sites. Specificity was 99.7%. Inhibitors were present more frequently in extrapulmonary than in pulmonary specimens (13.4% v 2.7%).
conclusionDespite the high sensitivity of the PCR assay for the diagnosis of tuberculosis in pulmonary specimens, it was less effective in the extrapulmonary samples. This is probably because of the lower bacterial load in extrapulmonary specimens, the presence of more inhibitors adversely affecting the PCR assay and the higher volume of specimens used for culture.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.