ArticleThe Journal of cell biology1996
Evidence using a green fluorescent protein-glucocorticoid receptor chimera that the Ran/TC4 GTPase mediates an essential function independent of nuclear protein import.
Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 156 citations in OpenAlex.
- B-type Plexins promote the GTPase activity of Ran to affect androgen receptor nuclear translocation in prostate cancer.Cancer gene therapy · 2023Article
- Ran promotes membrane targeting and stabilization of RhoA to orchestrate ovarian cancer cell invasion.Nature communications · 2019Article
- Mineralocorticoid Receptor May Regulate Glucose Homeostasis through the Induction of Interleukin-6 and Glucagon-Like peptide-1 in Pancreatic Islets.Journal of clinical medicine · 2019Article
- Mediated nuclear import and export of TAZ and the underlying molecular requirements.Nature communications · 2018Article
- mTOR complex 1 controls the nuclear localization and function of glycogen synthase kinase 3β.The Journal of biological chemistry · 2018Article
- ST8SIA4-Dependent Polysialylation is Part of a Developmental Program Required for Germ Layer Formation from Human Pluripotent Stem Cells.Stem cells (Dayton, Ohio) · 2016Article
- Altered transcription factor trafficking in oxidatively-stressed neuronal cells.Biochimica et biophysica acta · 2012Article
- Arginine topology controls escape of minimally cationic proteins from early endosomes to the cytoplasm.Chemistry & biology · 2012Article
- Relationship between protein intake and dynapenia in postmenopausal women.The journal of nutrition, health & aging · 2012Article
- Detection of dynamic spatiotemporal response to periodic chemical stimulation in a Xenopus embryonic tissue.PloS one · 2011Article
- Nuclear transport receptor goes moonlighting.Nature cell biology · 2010Article
- The ins and outs of nuclear trafficking: unusual aspects in apicomplexan parasites.DNA and cell biology · 2009Review
- RAN GTPase is an effector of the invasive/metastatic phenotype induced by osteopontin.Oncogene · 2008Article
- Functional analysis of key nuclear trafficking components reveals an atypical Ran network required for parasite pathogenesis.Molecular microbiology · 2008Article
- Characterization of nuclear import of the domain-specific androgen receptor in association with the importin alpha/beta and Ran-guanosine 5'-triphosphate systems.Endocrinology · 2008Article
- CAR and PXR: the xenobiotic-sensing receptors.Steroids · 2007Review
- The Ras-GRF1 exchange factor coordinates activation of H-Ras and Rac1 to control neuronal morphology.Molecular biology of the cell · 2006Article
- Charting biologically relevant chemical space: a structural classification of natural products (SCONP).Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Resolution of anaphase bridges in cancer cells.Chromosoma · 2004Article
- Paradoxical early glucocorticoid induction of stem cell factor (SCF) expression in inflammatory conditions.British journal of pharmacology · 2004Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The Ran/TC4 GTPase is required for the nuclear accumulation of artificial karyophiles in permeabilized cell assays. To investigate Ran function in a physiologically intact setting using mammalian cells, we examined the effects of several Ran mutants on cell growth and on the nuclear translocation of a glucocorticoid receptor-green fluorescent protein fusion (GR-GFP). Glucocorticoid receptor is cytosolic in the absence of ligand, but translocates to the nucleus on binding the agonist dexamethasone. After transfection into baby hamster kidney cells (BHK21), GR-GFP was detectable in living cells by direct fluorescence microscopy. Addition of dexamethasone caused a rapid translocation of the chimeric protein from the cytosol into the nucleus (t1/2 approximately 5 min). Cotransfection with epitope-tagged, wild-type Ran led to expression of HA1-Ran that was approximately 1.6-fold higher than the level of the endogenous protein, but it had no deleterious effect on nuclear import of the GR-GFP. However, expression of the Ran mutants G19V, T24N, or a COOH-terminal deletion (delta C) mutant dramatically reduced the accumulation of GR-GFP in the nuclei. An L43E mutant of Ran was without significant effect on nuclear GR-GFP import. Identical results were obtained following micro-injection of recombinant Ran mutants into cells expressing GR-GFP. Significantly, all of the Ran mutants, including L43E, strongly inhibited cell growth. These results demonstrate the use of GR-GFP in real-time imaging of nuclear transport. They also show that multiple types of Ran mutant exert dominant effects on this process, and that normal Ran function requires cycling between the GTP- and GDP-bound states of the protein. Most importantly, the results with the L43E Ran mutant provide strong evidence that Ran mediates a function essential to cell viability that is independent of nuclear protein import.
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