ArticleThe Journal of cell biology1996
A GTPase distinct from Ran is involved in nuclear protein import.
Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 50 citations in OpenAlex.
- Intersubunit Crosstalk in the Rag GTPase Heterodimer Enables mTORC1 to Respond Rapidly to Amino Acid Availability.Molecular cell · 2017Article
- Xanthine nucleotide-specific G-protein alpha-subunits: a novel approach for the analysis of G-protein-mediated signal transduction.Naunyn-Schmiedeberg's archives of pharmacology · 2004Review
- Induced nucleotide specificity in a GTPase.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Influence of cargo size on Ran and energy requirements for nuclear protein import.The Journal of cell biology · 2002Article
- Nucleocytoplasmic shuttling: a novel in vivo property of antisense phosphorothioate oligodeoxynucleotides.Nucleic acids research · 2000Article
- Review
- A monoclonal antibody to the COOH-terminal acidic portion of Ran inhibits both the recycling of Ran and nuclear protein import in living cells.The Journal of cell biology · 1999Article
- Rac-1 regulates nuclear factor of activated T cells (NFAT) C1 nuclear translocation in response to Fcepsilon receptor type 1 stimulation of mast cells.The Journal of experimental medicine · 1998Article
- Nuclear transport: run by Ran?American journal of human genetics · 1998Review
- Dbp5p/Rat8p is a yeast nuclear pore-associated DEAD-box protein essential for RNA export.The EMBO journal · 1998Article
- Transport of macromolecules between the nucleus and the cytoplasm.RNA (New York, N.Y.) · 1998Review
- Identification and functional characterization of a novel nuclear localization signal present in the yeast Nab2 poly(A)+ RNA binding protein.Molecular and cellular biology · 1998Article
- A T42A Ran mutation: differential interactions with effectors and regulators, and defect in nuclear protein import.Molecular biology of the cell · 1997Article
- Nucleocytoplasmic transport of macromolecules.Microbiology and molecular biology reviews : MMBR · 1997Review
- Importin alpha from Arabidopsis thaliana is a nuclear import receptor that recognizes three classes of import signals.Plant physiology · 1997Article
- Characterization of the nuclear protein import mechanism using Ran mutants with altered nucleotide binding specificities.The EMBO journal · 1996Article
- Identification of different roles for RanGDP and RanGTP in nuclear protein import.The EMBO journal · 1996Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Signal-dependent transport of proteins into the nucleus is a multi-step process mediated by nuclear pore complexes and cytosolic transport factors. One of the cytosolic factors, Ran, is the only GTPase that has a characterized role in the nuclear import pathway. We have used a mutant form of Ran with altered nucleotide binding specificity to investigate whether any other GTPases are involved in nuclear protein import. D125N Ran (XTP-Ran) binds specifically to xanthosine triphosphate (XTP) and has a greatly reduced affinity for GTP, so it is no longer sensitive to inhibition by nonhydrolyzable analogues of GTP such as guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S). using in vitro transport assays, we have found that nuclear import supported by XTP-Ran is nevertheless inhibited by the addition of non-hydrolyzable GTP analogues. This in conjunction with the properties of the inhibitory effect indicates that at least one additional GTPase is involved in the import process. Initial characterization suggests that the inhibited GTPase plays a direct role in protein import and could be a component of the nuclear pore complex.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.